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Neurofilament H antibody - 171 128

Neurofilaments are major neuronal intermediate filaments
Rabbit monoclonal recombinant IgG
Cat. No.: 171 128
Amount: 50 µg
Price: $415.00
Cat. No. 171 128 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
Clone RbN52
Subtype IgG1 (κ light chain)
Immunogen Full length purified pig Neurofilament H (UniProt Id: F1RFH3)
Reactivity Reacts with: mouse (P19246), rat (P16884), pig (F1RFH3), ape, human (P12036).
Other species not tested yet.
Specificity Detects phosphorylated and unphosphorylated Neurofilament H
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody N52. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 171_128.pdf
Cat. No.: 171 128
Amount: 50 µg
Price: $415.00
Background
Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.