Synaptic Systems - Neurofilament H

Cat. No.: 171 121

Amount: 100 µg

Price: $415.00

Cat. No. 171 121	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 100 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	N52
Subtype	IgG1
Immunogen	Full length purified pig Neurofilament H (UniProt Id: F1RFH3)
Reactivity	Reacts with: mouse (P19246), rat (P16884), pig (F1RFH3), ape, human (P12036). Other species not tested yet.
Specificity	Detects phosphorylated and unphosphorylated Neurofilament H.
Data sheet	171_121.pdf

References for Neurofilament H - 171 121

Expression of neurofilaments and of a titin epitope in thymic epithelial tumors. Implications for the pathogenesis of myasthenia gravis.
Marx A, Wilisch A, Schultz A, Greiner A, Magi B, Pallini V, Schalke B, Toyka K, Nix W, Kirchner T, Müller-Hermelink HK, et al.
The American journal of pathology (1996) 1486: 1839-50. 171 121 WB, IHC; tested species: human

Reactivity of a panel of neurofilament antibodies on phosphorylated and dephosphorylated neurofilaments.
Shaw G, Osborn M, Weber K
European journal of cell biology (1986) 421: 1-9. 171 121 IHC, WB; tested species: human,rat

Comparative study of the three neurofilament subunits within pig and human retinal ganglion cells.
Ruiz-Ederra J, García M, Hicks D, Vecino E
Molecular vision (2004) 10: 83-92. 171 121 IHC; tested species: pig

Evidence that Wallerian degeneration and localized axon degeneration induced by local neurotrophin deprivation do not involve caspases.
Finn JT, Weil M, Archer F, Siman R, Srinivasan A, Raff MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 204: 1333-41. 171 121 IHC; tested species: mouse,rat

Accumulation of amyloid beta and tau and the formation of neurofilament inclusions following diffuse brain injury in the pig.
Smith DH, Chen XH, Nonaka M, Trojanowski JQ, Lee VM, Saatman KE, Leoni MJ, Xu BN, Wolf JA, Meaney DF
Journal of neuropathology and experimental neurology (1999) 589: 982-92. 171 121 IHC; tested species: pig

Unexpected immunoreactivities of intermediate filament antibodies in human brain and brain tumors.
Franke FE, Schachenmayr W, Osborn M, Altmannsberger M
The American journal of pathology (1991) 1391: 67-79. 171 121 IHC; tested species: human

Evidence for a hepatocellular lineage in a combined hepatocellular-cholangiocarcinoma of transitional type.
Fisher HP, Doppl W, Osborn M, Altmannsberger M
Virchows Archiv. B, Cell pathology including molecular pathology (1988) 562: 71-6. 171 121 IHC; tested species: human

Cat. No.: 171 121

Amount: 100 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
4 ) Porchet R et al., Neuroreport (2003)
5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

ICC

IHC

IHC-P

Other products for this target

Neurofilament H antibody - 171 121

References for Neurofilament H - 171 121