Synaptic Systems - Neurofilament H

Cat. No.: 171 121

Amount: 100 µg

Price: $420.00

Cat. No. 171 121	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 100 up to 1 : 1000 gallery
Clone	N52
Subtype	IgG1
Immunogen	Full length purified pig Neurofilament H (UniProt Id: F1RFH3)
Reactivity	Reacts with: mouse (P19246), rat (P16884), pig (F1RFH3), ape, human (P12036). Other species not tested yet.
Specificity	Detects phosphorylated and unphosphorylated Neurofilament H.
Data sheet	171_121.pdf

References for Neurofilament H - 171 121

WB (2)
IHC (7)

Expression of neurofilaments and of a titin epitope in thymic epithelial tumors. Implications for the pathogenesis of myasthenia gravis.
Marx A, Wilisch A, Schultz A, Greiner A, Magi B, Pallini V, Schalke B, Toyka K, Nix W, Kirchner T, Müller-Hermelink HK, et al.
The American journal of pathology (1996) 1486: 1839-50. 171 121 WB, IHC; tested species: human

Reactivity of a panel of neurofilament antibodies on phosphorylated and dephosphorylated neurofilaments.
Shaw G, Osborn M, Weber K
European journal of cell biology (1986) 421: 1-9. 171 121 IHC, WB; tested species: human,rat

Comparative study of the three neurofilament subunits within pig and human retinal ganglion cells.
Ruiz-Ederra J, García M, Hicks D, Vecino E
Molecular vision (2004) 10: 83-92. 171 121 IHC; tested species: pig

Evidence that Wallerian degeneration and localized axon degeneration induced by local neurotrophin deprivation do not involve caspases.
Finn JT, Weil M, Archer F, Siman R, Srinivasan A, Raff MC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2000) 204: 1333-41. 171 121 IHC; tested species: mouse,rat

Accumulation of amyloid beta and tau and the formation of neurofilament inclusions following diffuse brain injury in the pig.
Smith DH, Chen XH, Nonaka M, Trojanowski JQ, Lee VM, Saatman KE, Leoni MJ, Xu BN, Wolf JA, Meaney DF
Journal of neuropathology and experimental neurology (1999) 589: 982-92. 171 121 IHC; tested species: pig

Unexpected immunoreactivities of intermediate filament antibodies in human brain and brain tumors.
Franke FE, Schachenmayr W, Osborn M, Altmannsberger M
The American journal of pathology (1991) 1391: 67-79. 171 121 IHC; tested species: human

Evidence for a hepatocellular lineage in a combined hepatocellular-cholangiocarcinoma of transitional type.
Fisher HP, Doppl W, Osborn M, Altmannsberger M
Virchows Archiv. B, Cell pathology including molecular pathology (1988) 562: 71-6. 171 121 IHC; tested species: human

Cat. No.: 171 121

Amount: 100 µg

Price: $420.00

References
WB (2)
IHC (7)

ICC

IHC

IHC-P

Background

Neurofilaments (NFs) are intermediate filaments essential for providing structural support to neurons, particularly within axons. They play a crucial role in maintaining axonal diameter, which directly influences nerve conduction velocity (1). Neurofilaments are composed of three primary subunits - NF-L (light), NF-M (medium) and NF-H (heavy) – along with an NF-associated protein. In the adult central nervous system (CNS), α-internexin serves as the fourth neurofilament subunit, whereas in the peripheral nervous system (PNS), peripherin takes on this role (2).
Beyond their structural function, neurofilaments are also valuable biomarkers in both research and clinical settings. They are widely used in immunohistochemistry to stain and visualize axons, particularly in peripheral nerves and the CNS. Increased levels of neurofilament proteins in cerebrospinal fluid (CSF) or blood are strongly associated with neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), multiple sclerosis (MS), and Alzheimer’s disease (3). In peripheral nerve studies, neurofilament staining is often combined with other markers, such as S100, to provide a more comprehensive assessment of nerve structure and pathology (4).

1 ) Rao MV et al., J Cell Biol (1998)
2 ) Gafson AR et al., Brain (2020)
3 ) Yuan A et al., Front Neurosci (2021)
4 ) Hernandez S et al., Adv Biol (Weinh) (2022)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

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Neurofilament H antibody - 171 121

References for Neurofilament H - 171 121