Neurofilament L antibody - 171 014

Neurofilaments are major neuronal intermediate filaments

Guinea pig polyclonal antiserum

Cat. No.: 171 014

Amount: 100 µl

Price: $365.00

Cat. No. 171 014	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: gallery IHC-P: 1 : 250 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Neurofilament L. (UniProt Id: P19527)
Reactivity	Reacts with: mouse (P08551), rat (P19527). Other species not tested yet.
Data sheet	171_014.pdf

References for Neurofilament L - 171 014

Neuraminidase inhibition promotes the collective migration of neurons and recovery of brain function.
Matsumoto M, Matsushita K, Hane M, Wen C, Kurematsu C, Ota H, Bang Nguyen H, Quynh Thai T, Herranz-Pérez V, Sawada M, Fujimoto K, et al.
EMBO molecular medicine (2024) : . 171 014 IHC; tested species: mouse

Cat. No.: 171 014

Amount: 100 µl

Price: $365.00

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
4 ) Porchet R et al., Neuroreport (2003)
5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

Standard protocol

ICC

IHC-P

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