Synaptic Systems - alpha-Internexin

Cat. No.: 167 003

Amount: 50 µg

Price: $460.00

Alpha-Internexin (red) and Neurofilament L (green) staining in a mouse hippocampus section

WB

ICC

IHC

IHC-P

IHC-Fr

Background

The cytoskeleton of most eukaryotic cells is composed of three distinct components: Actin-based microfilaments, tubulin based microtubules and intermediate filaments (IFs).
α-Internexin is a neuronal intermediate filament of type four. It is assumed to be expressed by all neurons and preceeds the onset of the expression of the heavy, medium and light variants of neurofilaments which are major components of the neuronal IFs.
Alterations in the phosphorylation state of IFs have been associated with neurodegenerative diseases like Alzheimer, Parkinson, dementia with Lewy bodies (DLB), and motor neuron disease (MND).

1 ) Yuan A et al., J. Neurosci. (2006)
2 ) Armstrong RA et al., Eur. J. Neurol. (2006)
3 ) Ko TL et al., J. Biomed. Sci. (2005)
4 ) Chien CL et al., J. Neurosci. Res. (2005)
5 ) Levavasseur F et al., Brain Res. Mol. Brain Res. (1999)
6 ) Ching GY et al., J. Neurosci. (1999)
7 ) Abumuhor IA et al., J. Struct. Biol. (1998)
8 ) Suzuki T et al., Brain Res. (1997)
9 ) Benson DL et al., J. Neurocytol. (1996)
10 ) Tanaka J et al., Biochem. Biophys. Res. Commun. (1993)

Protocols

WB

ICC

Fixed Cells

IHC

IHC-P

IHC-Fr

Other products for this target

Cat. No. 167 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 200 (see remarks) gallery IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full-length recombinant human α-Internexin (UniProt Id: Q16352)
Reactivity	Reacts with: mouse (P46660), rat (P23565), human (Q16352). Other species not tested yet.
Remarks	ICC: Methanol fixation is recommended. The following fixatives are not advised: 4% formaldehyde/PFA. IHC: Antigen retrieval with citrate buffer pH 6 is required. IHC-P: Antigen retrieval with Tris-EDTA buffer pH 9 is recommended for chromogenic detection. IHC-Fr: Acetone fixation is recommended.
Data sheet	167_003.pdf

alpha-Internexin antibody - 167 003