Synaptic Systems - Synaptopodin

Cat. No.: 163 004

Amount: 100 µl

Price: $375.00

Cat. No. 163 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 gallery IHC-P: 1 : 200 up to 1 : 500 gallery ExM: 1 : 100
Immunogen	Recombinant protein corresponding to residues near the central region of mouse Synaptopodin. (UniProt Id: Q8CC35)
Reactivity	Reacts with: rat (Q9Z327), mouse (Q8CC35), human (Q8N3V7). Other species not tested yet.
Specificity	This antibody detects the renal Synpo-long and the neuronal Synpo-short isofoms but is negativ for the T-variant. K.O. validated PubMed: 28922860
Matching control protein/peptide	163-0P
Remarks	WB: The antibody is less sensitive than the rabbit antiserum (cat. no. 163 002).
Data sheet	163_004.pdf

References for Synaptopodin - 163 004

WB (1)
ICC (4)
IHC (11)
IHC-P (5)
ExM (1)

Smad4 promotes diabetic nephropathy by modulating glycolysis and OXPHOS.
Li J, Sun YBY, Chen W, Fan J, Li S, Qu X, Chen Q, Chen R, Zhu D, Zhang J, Wu Z, et al.
EMBO reports (2020) : e48781. 163 004 WB, IHC; tested species: mouse

Stabilization of Spine Synaptopodin by mGluR1 Is Required for mGluR-LTD.
Speranza L, Inglebert Y, De Sanctis C, Wu PY, Kalinowska M, McKinney RA, Francesconi A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 429: 1666-1678. 163 004 ICC; tested species: mouse

An ex vivo culture model of kidney podocyte injury reveals mechanosensitive, synaptopodin-templating, sarcomere-like structures.
Jiang S, Alisafaei F, Huang YY, Hong Y, Peng X, Qu C, Puapatanakul P, Jain S, Miner JH, Genin GM, Suleiman HY, et al.
Science advances (2022) 835: eabn6027. 163 004 ICC; tested species: human,mouse

Synaptopodin Is Dispensable for Normal Podocyte Homeostasis but Is Protective in the Context of Acute Podocyte Injury.
Ning L, Suleiman HY, Miner JH
Journal of the American Society of Nephrology : JASN (2020) 3112: 2815-2832. 163 004 ICC; KO verified; tested species: mouse

The Role of Synaptopodin in Membrane Protein Diffusion in the Dendritic Spine Neck.
Wang L, Dumoulin A, Renner M, Triller A, Specht CG
PloS one (2016) 112: e0148310. 163 004 ICC

The proteasome modulates endocytosis specifically in glomerular cells to promote kidney filtration.
Sachs W, Blume L, Loreth D, Schebsdat L, Hatje F, Koehler S, Wedekind U, Sachs M, Zieliniski S, Brand J, Conze C, et al.
Nature communications (2024) 151: 1897. 163 004 IHC; tested species: mouse

Maintenance of Lognormal-Like Skewed Dendritic Spine Size Distributions in Dentate Granule Cells of TNF, TNF-R1, TNF-R2, and TNF-R1/2-Deficient Mice.
Rößler N, Smilovic D, Vuksic M, Jedlicka P, Deller T
The Journal of comparative neurology (2024) 5327: e25645. 163 004 IHC; tested species: mouse

DOT1L protects against podocyte injury in diabetic kidney disease through phospholipase C-like 1.
Hu Y, Ye S, Kong J, Zhou Q, Wang Z, Zhang Y, Yan H, Wang Y, Li T, Xie Y, Chen B, et al.
Cell communication and signaling : CCS (2024) 221: 519. 163 004 IHC; tested species: mouse

Loss of tumor necrosis factor (TNF)-receptor 1 and TNF-receptor 2 partially replicate effects of TNF deficiency on dendritic spines of granule cells in mouse dentate gyrus.
Smilovic D, Rietsche M, Fellenz M, Drakew A, Vuksic M, Deller T
The Journal of comparative neurology (2023) 5312: 281-293. 163 004 IHC; tested species: mouse

Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome.
Speranza L, Filiz KD, Goebel S, Perrone-Capano C, Pulcrano S, Volpicelli F, Francesconi A
Biomedicines (2022) 1011: . 163 004 IHC; tested species: mouse

Asparaginyl endopeptidase protects against podocyte injury in diabetic nephropathy through cleaving cofilin-1.
Lei C, Li M, Qiu Y, Xie Y, Hao Z, Yin X, Zhang Z, Su H, Yang L, Lin J, Hammes HP, et al.
Cell death & disease (2022) 132: 184. 163 004 IHC; tested species: mouse

Constitutive tumor necrosis factor (TNF)-deficiency causes a reduction in spine density in mouse dentate granule cells accompanied by homeostatic adaptations of spine head size.
Smilovic D, Rietsche M, Drakew A, Vuksic M, Deller T
The Journal of comparative neurology (2021) : . 163 004 IHC; tested species: mouse

Anaerobic Glycolysis Maintains the Glomerular Filtration Barrier Independent of Mitochondrial Metabolism and Dynamics.
Brinkkoetter PT, Bork T, Salou S, Liang W, Mizi A, Özel C, Koehler S, Hagmann HH, Ising C, Kuczkowski A, Schnyder S, et al.
Cell reports (2019) 275: 1551-1566.e5. 163 004 IHC; tested species: mouse

Granule Cell Ensembles in Mouse Dentate Gyrus Rapidly Upregulate the Plasticity-Related Protein Synaptopodin after Exploration Behavior.
Paul MH, Choi M, Schlaudraff J, Deller T, Del Turco D
Cerebral cortex (New York, N.Y. : 1991) (2019) : . 163 004 IHC; tested species: mouse

Structural Plasticity of Synaptopodin in the Axon Initial Segment during Visual Cortex Development.
Schlüter A, Del Turco D, Deller T, Gutzmann A, Schultz C, Engelhardt M
Cerebral cortex (New York, N.Y. : 1991) (2017) 279: 4662-4675. 163 004 IHC; KO verified

Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens.
Kylies D, Zimmermann M, Haas F, Schwerk M, Kuehl M, Brehler M, Czogalla J, Hernandez LC, Konczalla L, Okabayashi Y, Menzel J, et al.
Nature nanotechnology (2023) : . 163 004 IHC-P, ExM; tested species: human,mouse

The classical pathway triggers pathogenic complement activation in membranous nephropathy.
Seifert L, Zahner G, Meyer-Schwesinger C, Hickstein N, Dehde S, Wulf S, Köllner SMS, Lucas R, Kylies D, Froembling S, Zielinski S, et al.
Nature communications (2023) 141: 473. 163 004 IHC-P; tested species: mouse

Th17 cell plasticity towards a T-bet-dependent Th1 phenotype is required for bacterial control in Staphylococcus aureus infection.
Bartsch P, Kilian C, Hellmig M, Paust HJ, Borchers A, Sivayoganathan A, Enk L, Zhao Y, Shaikh N, Büttner H, Wong MN, et al.
PLoS pathogens (2022) 184: e1010430. 163 004 IHC-P; tested species: mouse

SMPDL3b modulates radiation-induced DNA damage response in renal podocytes.
Francis M, Ahmad A, Bodgi L, Azzam P, Youssef T, Abou Daher A, Eid AA, Fornoni A, Pollack A, Marples B, Zeidan YH, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022) 3610: e22545. 163 004 IHC-P; tested species: mouse

Podocyte endowment and the impact of adult body size on kidney health.
Cullen-McEwen LA, van der Wolde J, Haruhara K, Tribolet L, Dowling JP, Bertram MG, de Matteo R, Haas F, Czogalla J, Okabayashi Y, Armitage JA, et al.
American journal of physiology. Renal physiology (2021) 3213: F322-F334. 163 004 IHC-P; tested species: mouse

Cat. No.: 163 004

Amount: 100 µl

Price: $375.00

References
WB (1)
ICC (4)
IHC (11)
IHC-P (5)
ExM (1)

The Role of Synaptopodin in Membrane Protein Diffusion in the Dendritic Spine Neck.
Wang L, Dumoulin A, Renner M, Triller A, Specht CG
PloS one (2016) 112: e0148310. 163 004 ICC

ICC

IHC

IHC-P

Background

Synaptopodin is the founding member of a class of proline-rich actin-associated proteins and has been originally identified in podocytes of renal glomeruli (1, 2). In brain it localizes to the post-synaptic density (PSD) and the spine aparatus (1). In humans, three isoforms of synaptopodin with a distinct expression profile have been identified, neuronal Synpo-short, renal Synpo-long and Synpo-T. All three isoforms interact with alpha-actinin and induce alpha-actinin-induced actin filaments (3).
Synaptopodin deficient mice lack the dendritic spine apparatus and exhibit impaired activity-dependent long-term synaptic plasticity (4).

1 ) Mundel P et al., J. Cell Biol. (1997)
2 ) Mundel P et al., J. Histochem. Cytochem. (1991)
3 ) Asanuma K et al., J. Clin. Invest. (2005)
4 ) Deller T et al., Proc. Natl. Acad. Sci. U.S.A. (2003)

Protocols

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

OTHER

Preabsorbtion

Other products for this target

Synaptopodin antibody - 163 004 K.O.

References for Synaptopodin - 163 004