|Cat. No. 162 211||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: not recommended
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 (see remarks) gallery
IHC-P/FFPE: not recommended
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1042 to 1475 from rat Shank2 (UniProt Id: Q9QX74)|
Epitop: AA 1042 to 1475 from rat Shank2 (UniProt Id: Q9QX74)
Reacts with: rat (Q9QX74).
Other species not tested yet.
|Specificity||Specific for Shank 2.|
IHC: Fix for 15 min with 4% PFA and 15% picric acid in PBS.
Shank 1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP 1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.