Shank2 (SPANK3) antibody - 162 208

Shanks are multidomain proteins of the postsynaptic density

Rabbit monoclonal recombinant IgG

Cat. No.: 162 208

Amount: 50 µg

Price: $420.00

Cat. No. 162 208	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC-P (FFPE): not recommended IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb298F5
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat Shank2 (UniProt Id: Q9QX74)
Reactivity	Reacts with: mouse (Q80Z38), rat (Q9QX74). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 298F5. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC-Fr: The following fixatives are possible: acetone, 4% formaldehyde/PFA. Methanol fixation is not advised.
Data sheet	Data Sheet 162_208 Safety Data Sheet (SDS)

Cat. No.: 162 208

Amount: 50 µg

Price: $420.00

Shank2 (red) staining in fresh frozen murine hippocampus and cerebellum

ICC
(1 img)

IHC-Fr
(1 img)

Background

Shank1, 2 and 3 are major proteins of the postsynaptic density (PSD). They are composed of several protein-protein interaction domains like PDZ-, homer- and ABP1-binding domains which allow them to crosslink ionotopic and metabotropic glutamate receptor complexes with each other and to the actin-cytoskeleton.

1 ) Sala C et al., J. Neurosci. (2005)
2 ) Roussignol G et al., J. Neurosci. (2005)
3 ) Quitsch A et al., J. Neurosci. (2005)
4 ) Qualmann B et al., J. Neurosci. (2004)
5 ) Im YJ et al., J. Biol. Chem. (2003)
6 ) Boeckers TM et al., J. Neurochem. (2002)
7 ) Sala C et al., Neuron (2001)
8 ) Tobaben S et al., J. Biol. Chem. (2000)
9 ) Sheng M et al., J. Cell. Sci. (2000)
10 ) Naisbitt S et al., Neuron (1999)

Protocols

ICC

Fixed Cells

IHC-Fr

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