Tailor-made Antibodies
and Tools for Life Science

Shank2 antibody - 162 202 K.O.

Shanks are multidomain proteins of the postsynaptic density
Rabbit polyclonal antiserum
Cat. No.: 162 202
Amount: 200 µl
Price: $360.00
Cat. No. 162 202 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Shank2 (UniProt Id: Q9QX74)
Reactivity Reacts with: rat (Q9QX74), mouse (Q80Z38).
Other species not tested yet.
Specificity K.O. validated PubMed: 29572432
Remarks

IHC: This antibody requires mild fixation with 1% PFA at low pH (pH 6.0).
IHC-Fr: Fixation with acetone or PFA/formaldehyde is recommended. Postfixation with methanol is not advised.

Data sheet 162_202.pdf

References for Shank2 - 162 202

CSF1R-mediated myeloid cell depletion shifts the ratio of motor cortical excitatory to inhibitory neurons in a multiple system atrophy model.
Gauer C, Battis K, Schneider Y, Florio JB, Mante M, Kim HY, Rissman RA, Hoffmann A, Winkler J
Experimental neurology (2024) 374: 114706. 162 202 WB; tested species: mouse
Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspring.
Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A, Peano C, et al.
Immunity (2021) 5411: 2611-2631.e8. 162 202 WB; tested species: mouse
Insulin-like growth factor 1 partially rescues early developmental defects caused by SHANK2 knockdown in human neurons.
Chen ST, Lai WJ, Zhang WJ, Chen QP, Zhou LB, So KF, Shi LL
Neural regeneration research (2020) 1512: 2335-2343. 162 202 WB, ICC; KD verified; tested species: human
Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 162 202 WB; tested species: mouse
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Filipello F, Morini R, Corradini I, Zerbi V, Canzi A, Michalski B, Erreni M, Markicevic M, Starvaggi-Cucuzza C, Otero K, Piccio L, et al.
Immunity (2018) : . 162 202 WB; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 162 202 WB; tested species: mouse
Early Exposure to a High-Fat Diet Impacts on Hippocampal Plasticity: Implication of Microglia-Derived Exosome-like Extracellular Vesicles.
Vinuesa A, Bentivegna M, Calfa G, Filipello F, Pomilio C, Bonaventura MM, Lux-Lantos V, Matzkin ME, Gregosa A, Presa J, Matteoli M, et al.
Molecular neurobiology (2018) : . 162 202 WB; tested species: mouse
Cell-Type-Specific Shank2 Deletion in Mice Leads to Differential Synaptic and Behavioral Phenotypes.
Kim R, Kim J, Chung C, Ha S, Lee S, Lee E, Yoo YE, Kim W, Shin W, Kim E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3817: 4076-4092. 162 202 WB, IHC; KO verified; tested species: mouse
Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice.
Lee S, Lee E, Kim R, Kim J, Lee S, Park H, Yang E, Kim H, Kim E
Frontiers in molecular neuroscience (2018) 11: 209. 162 202 WB; KO verified; tested species: mouse
Cerebellar Shank2 Regulates Excitatory Synapse Density, Motor Coordination, and Specific Repetitive and Anxiety-Like Behaviors.
Ha S, Lee D, Cho YS, Chung C, Yoo YE, Kim J, Lee J, Kim W, Kim H, Bae YC, Tanaka-Yamamoto K, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3648: 12129-12143. 162 202 WB, IHC; KO verified; tested species: mouse
REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 162 202 WB
Cat. No.: 162 202
Amount: 200 µl
Price: $360.00