Arc antibody - 156 011

Arc is a cytoskeleton-associated protein involved in neuronal plasticity

Mouse monoclonal purified IgG

Cat. No.: 156 011

Amount: 100 µg

Price: $415.00

Cat. No. 156 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	48B2
Subtype	IgG2b (κ light chain)
Immunogen	Full-length recombinant mouse Arc (UniProt Id: Q9WV31)
Epitop	AA 1 to 214 from mouse Arc (UniProt Id: Q9WV31)
Reactivity	Reacts with: rat (Q63053), mouse (Q9WV31). Other species not tested yet.
Specificity	Specific for Arc.
Remarks	ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 156 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	156_011.pdf

References for Arc - 156 011

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 156 011 ELISA

Cat. No.: 156 011

Amount: 100 µg

Price: $415.00

Background

Immediate-early genes (IEGs) are rapidely induced after patterned synaptic activity. Genes that are involved in this complex response code for transcription and growth factors, metabolic and signaling enzymes, small GTP binding proteins and structural proteins. Some of these proteins may play a crucial role in long term plasticity which is important for learning processes.
The activity regulated cytoskeleton associated protein Arc or Arg 3.1 is enriched in dendrites and colocalizes with F-Actin. Direct interaction of Arc with actin has also been demonstrated by biochemical studies.

1 ) Larsen MH et al., Brain Res. (2005)
2 ) McIntyre CK et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
3 ) Chawla MK et al., Hippocampus (2005)
4 ) Vazdarjanova A et al., J. Neurosci. (2002)
5 ) Guzowski JF et al., J. Neurosci. (2000)
6 ) Tan A et al., J. Neurochem. (2000)
7 ) Guzowski JF et al., Nat. Neurosci. (1999)
8 ) Steward O et al., Neuron (1998)
9 ) Lyford GL et al., Neuron (1995)

Protocols

Standard protocol

ELISA

Membrane proteins

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