Synaptic Systems

Cat. No.: 156 008

Amount: 50 µg

Price: $415.00

Cat. No. 156 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb150H10
Subtype	IgG1 (κ light chain)
Immunogen	Full length recombinant mouse Arc (UniProt Id: Q9WV31)
Reactivity	Reacts with: mouse (Q9WV31), rat (Q63053). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-150H10. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	156_008.pdf

References for Arc - 156 008

MicroRNA-33 regulates the synaptic plasticity-related gene ARC in temporal lobe epilepsy.
Xu Y, Zhang L, Yan Y, Xiao W, Zou W, Luo Z, Xiao B, Long H
Neuroscience research (2024) : . 156 008 WB, IHC; tested species: human,rat

Cat. No.: 156 008

Amount: 50 µg

Price: $415.00

ICC

IHC

IHC-P

Background

Immediate-early genes (IEGs) are rapidely induced after patterned synaptic activity. Genes that are involved in this complex response code for transcription and growth factors, metabolic and signaling enzymes, small GTP binding proteins and structural proteins. Some of these proteins may play a crucial role in long term plasticity which is important for learning processes.
The activity regulated cytoskeleton associated protein Arc or Arg 3.1 is enriched in dendrites and colocalizes with F-Actin. Direct interaction of Arc with actin has also been demonstrated by biochemical studies.

1 ) Larsen MH et al., Brain Res. (2005)
2 ) McIntyre CK et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
3 ) Chawla MK et al., Hippocampus (2005)
4 ) Vazdarjanova A et al., J. Neurosci. (2002)
5 ) Guzowski JF et al., J. Neurosci. (2000)
6 ) Tan A et al., J. Neurochem. (2000)
7 ) Guzowski JF et al., Nat. Neurosci. (1999)
8 ) Steward O et al., Neuron (1998)
9 ) Lyford GL et al., Neuron (1995)

Protocols

ICC

IHC

IHC-P

Other products for this target

Arc antibody - 156 008

References for Arc - 156 008