Synaptic Systems

Cat. No.: 156 005

Amount: 100 µl

Price: $465.00

Cat. No. 156 005	100 µl specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full-length recombinant mouse Arc (UniProt Id: Q9WV31)
Reactivity	Reacts with: rat (Q63053), mouse (Q9WV31). Other species not tested yet.
Specificity	Specific for arc. K.O. validated
Data sheet	Datasheet 156_005

References for Arc - 156 005

ARC/ARG3.1 binds the nuclear polyadenylate-binding protein RRM and regulates neuronal activity-dependent formation of nuclear speckles.
Kanhema T, Parobczak K, Patil S, Holm-Kaczmarek D, Hallin EI, Ludwiczak J, Szczepankiewicz AA, Pauzin FP, Mahboob A, Szum A, Ishizuka Y, et al.
Cell reports (2025) 444: 115525. 156 005 WB, IHC; tested species: rat

Class I Histone Deacetylase Inhibition by Tianeptinaline Modulates Neuroplasticity and Enhances Memory.
Zhao WN, Ghosh B, Tyler M, Lalonde J, Joseph NF, Kosaric N, Fass DM, Tsai LH, Mazitschek R, Haggarty SJ
ACS chemical neuroscience (2018) : . 156 005 ICC; tested species: mouse

Sevoflurane-induced disruption of critical period Arc signaling drives aberrant microglial synaptic pruning and cognitive deficits.
Chen BH, Chen YR, Zheng LY, Cai HJ, Ke XL, Li SY, Chen ZJ, Zhang XN, Zhou FQ, Chen G
Acta pharmacologica Sinica (2026) : . 156 005 IHC; tested species: mouse

Identification of an engram ensemble mediating memory forgetting in the dentate gyrus.
Hu S, Yang J, Yin S, Zhong Y, An Y, Guo Y, Zhai Z, Zhong Y
Neuron (2026) 1144: 759-773.e5. 156 005 IHC; tested species: mouse

Combinative Protein Expression of Immediate Early Genes c-Fos, Arc, and Npas4 Along Aversive and Appetitive Experience-Related Neural Networks.
Arai M, Osanai H, Snell CC, Gawf KE, Kitamura T, Ogawa SK
Hippocampus (2025) 355: e70030. 156 005 IHC; tested species: mouse

Neuronal activity-related transcription is blunted in immature compared to mature dentate granule cells.
Parylak SL, Qiu F, Linker SB, Gallina IS, Lim CK, Preciado D, McDonald AH, Zhou X, Gage FH
Hippocampus (2023) 334: 412-423. 156 005 IHC; tested species: mouse

A novel environment-evoked transcriptional signature predicts reactivity in single dentate granule neurons.
Jaeger BN, Linker SB, Parylak SL, Barron JJ, Gallina IS, Saavedra CD, Fitzpatrick C, Lim CK, Schafer ST, Lacar B, Jessberger S, et al.
Nature communications (2018) 91: 3084. 156 005 IHC; tested species: mouse

Distinct synaptic and neurochemical changes to the granule cell-CA3 projection in Bassoon mutant mice.
Dieni S, Nestel S, Sibbe M, Frotscher M, Hellwig S
Frontiers in synaptic neuroscience (2015) 7: 18. 156 005 IHC; tested species: mouse

Immature doublecortin-positive hippocampal neurons are important for learning but not for remembering.
Vukovic J, Borlikova GG, Ruitenberg MJ, Robinson GJ, Sullivan RK, Walker TL, Bartlett PF
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3315: 6603-13. 156 005 IHC; tested species: mouse

BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu MS, Deogracias R, Gundelfinger ED, Kojima M, Nestel S, Frotscher M, Barde YA, et al.
The Journal of cell biology (2012) 1966: 775-88. 156 005 IHC

Cat. No.: 156 005

Amount: 100 µl

Price: $465.00

WB
(1 img)

ICC
(1 img)

IHC
(1 img)

Background

Immediate-early genes (IEGs) are rapidly induced after patterned synaptic activity. Genes that are involved in this complex response code for transcription and growth factors, metabolic and signaling enzymes, small GTP binding proteins and structural proteins. Some of these proteins may play a crucial role in long term plasticity which is important for learning processes.
The activity regulated cytoskeleton associated protein Arc or Arg 3.1 is enriched in dendrites and colocalizes with F-Actin. Direct interaction of Arc with actin has also been demonstrated by biochemical studies.

1 ) Larsen MH et al., Brain Res. (2005)
2 ) McIntyre CK et al., Proc. Natl. Acad. Sci. U.S.A. (2005)
3 ) Chawla MK et al., Hippocampus (2005)
4 ) Vazdarjanova A et al., J. Neurosci. (2002)
5 ) Guzowski JF et al., J. Neurosci. (2000)
6 ) Tan A et al., J. Neurochem. (2000)
7 ) Guzowski JF et al., Nat. Neurosci. (1999)
8 ) Steward O et al., Neuron (1998)
9 ) Lyford GL et al., Neuron (1995)

Protocols

ICC

Fixed Cells

IHC

Other products for this target

Arc antibody - 156 005 K.O.

References for Arc - 156 005