Tailor-made Antibodies
and Tools for Life Science

Ca2+ channel T-type alpha-1G antibody - 152 503

Rabbit polyclonal purified antibody
Cat. No.: 152 503
Amount: 50 µg
Price: $380.00
Cat. No. 152 503 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1921 to 2254 from rat Ca2+ channel T-type α-1G (Cav3.1) (UniProt Id: O54898)
Reactivity Reacts with: rat (O54898), mouse (Q9WUT2).
Other species not tested yet.
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel T-type alpha-1G aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 152_503.pdf

References for Ca2+ channel T-type alpha-1G - 152 503

Homeostatic plasticity and burst activity are mediated by hyperpolarization-activated cation currents and T-type calcium channels in neuronal cultures.
Rátkai A, Tárnok K, Aouad HE, Micska B, Schlett K, Szücs A
Scientific reports (2021) 111: 3236. 152 503 WB; tested species: mouse
Cat. No.: 152 503
Amount: 50 µg
Price: $380.00