Cat. No.: 152 403
Amount: 50 µg
Price:
$380.00
|
|
|
| Cat. No. 152 403 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to AA 1921 to 2222 from rat Ca2+ channel R-type α-1E (Cav2.3) (UniProt Id: Q07652) |
| Reactivity |
Reacts with: rat (Q07652), mouse (Q61290). Other species not tested yet. |
| Remarks |
WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel R-type alpha-1E aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: |
| Data sheet | 152_403.pdf |
|
|