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Ca2+ channel P/Q-type alpha-1A antibody - 152 205

Guinea pig polyclonal purified antibody
Cat. No.: 152 205
Amount: 50 µg
Price: $465.00
Cat. No. 152 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
EM: yes gallery  
Immunogen Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P54282)
Reactivity Reacts with: rat (P54282), mouse (P97445).
Other species not tested yet.
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel P/Q-type alpha-1A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: This antibody requires mild fixation.

Data sheet 152_205.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 205

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 152 205 ICC; tested species: rat
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 152 205 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 152 205 IHC; tested species: rat
Correct expression and localization of collagen xiii is crucial for the normal formation and function of the neuromuscular system.
Härönen H, Zainul Z, Naumenko N, Sormunen R, Miinalainen I, Shakirzyanova A, Santoleri S, Kemppainen AV, Giniatullin R, Pihlajaniemi T, Heikkinen A, et al.
The European journal of neuroscience (2019) : . 152 205 IHC; tested species: mouse
RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 152 205 IHC; tested species: mouse
Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
Chen JJ, Kaufmann WA, Chen C, Arai I, Kim O, Shigemoto R, Jonas P
Neuron (2024) 1125: 755-771.e9. 152 205 EM; tested species: mouse
Nanoscale Phosphoinositide Distribution on Cell Membranes of Mouse Cerebellar Neurons.
Eguchi K, Le Monnier E, Shigemoto R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4323: 4197-4216. 152 205 EM; tested species: mouse
Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images.
Guerrero-Given D, Goldin SL, Thomas CI, Anthony SA, Jerez D, Kamasawa N
Frontiers in neuroanatomy (2022) 16: 855218. 152 205 EM; tested species: mouse
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 152 205 EM; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 152 205 EM; tested species: mouse
Presynaptic development is controlled by the core active zone proteins CAST/ELKS.
Radulovic T, Dong W, Goral RO, Thomas CI, Veeraraghavan P, Montesinos MS, Guerrero-Given D, Goff K, Lübbert M, Kamasawa N, Ohtsuka T, et al.
The Journal of physiology (2020) 59812: 2431-2452. 152 205 EM; tested species: mouse
CAST/ELKS Proteins Control Voltage-Gated Ca2+ Channel Density and Synaptic Release Probability at a Mammalian Central Synapse.
Dong W, Radulovic T, Goral RO, Thomas C, Suarez Montesinos M, Guerrero-Given D, Hagiwara A, Putzke T, Hida Y, Abe M, Sakimura K, et al.
Cell reports (2018) 242: 284-293.e6. 152 205 EM; tested species: mouse
CaV2.1 α1 Subunit Expression Regulates Presynaptic CaV2.1 Abundance and Synaptic Strength at a Central Synapse.
Lübbert M, Goral RO, Keine C, Thomas C, Guerrero-Given D, Putzke T, Satterfield R, Kamasawa N, Young SM
Neuron (2018) : . 152 205 EM; tested species: mouse
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 152 205 EM
Inhibitory and excitatory axon terminals share a common nano-architecture of their Cav2.1 (P/Q-type) Ca(2+) channels.
Althof D, Baehrens D, Watanabe M, Suzuki N, Fakler B, Kulik Á
Frontiers in cellular neuroscience (2015) 9: 315. 152 205 EM
Cat. No.: 152 205
Amount: 50 µg
Price: $465.00
Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 152 205 ICC; tested species: rat
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 152 205 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 152 205 IHC; tested species: rat
Correct expression and localization of collagen xiii is crucial for the normal formation and function of the neuromuscular system.
Härönen H, Zainul Z, Naumenko N, Sormunen R, Miinalainen I, Shakirzyanova A, Santoleri S, Kemppainen AV, Giniatullin R, Pihlajaniemi T, Heikkinen A, et al.
The European journal of neuroscience (2019) : . 152 205 IHC; tested species: mouse
RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 152 205 IHC; tested species: mouse
Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
Chen JJ, Kaufmann WA, Chen C, Arai I, Kim O, Shigemoto R, Jonas P
Neuron (2024) 1125: 755-771.e9. 152 205 EM; tested species: mouse
Nanoscale Phosphoinositide Distribution on Cell Membranes of Mouse Cerebellar Neurons.
Eguchi K, Le Monnier E, Shigemoto R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4323: 4197-4216. 152 205 EM; tested species: mouse
Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images.
Guerrero-Given D, Goldin SL, Thomas CI, Anthony SA, Jerez D, Kamasawa N
Frontiers in neuroanatomy (2022) 16: 855218. 152 205 EM; tested species: mouse
Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 152 205 EM; tested species: mouse
Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 152 205 EM; tested species: mouse
Presynaptic development is controlled by the core active zone proteins CAST/ELKS.
Radulovic T, Dong W, Goral RO, Thomas CI, Veeraraghavan P, Montesinos MS, Guerrero-Given D, Goff K, Lübbert M, Kamasawa N, Ohtsuka T, et al.
The Journal of physiology (2020) 59812: 2431-2452. 152 205 EM; tested species: mouse
CAST/ELKS Proteins Control Voltage-Gated Ca2+ Channel Density and Synaptic Release Probability at a Mammalian Central Synapse.
Dong W, Radulovic T, Goral RO, Thomas C, Suarez Montesinos M, Guerrero-Given D, Hagiwara A, Putzke T, Hida Y, Abe M, Sakimura K, et al.
Cell reports (2018) 242: 284-293.e6. 152 205 EM; tested species: mouse
CaV2.1 α1 Subunit Expression Regulates Presynaptic CaV2.1 Abundance and Synaptic Strength at a Central Synapse.
Lübbert M, Goral RO, Keine C, Thomas C, Guerrero-Given D, Putzke T, Satterfield R, Kamasawa N, Young SM
Neuron (2018) : . 152 205 EM; tested species: mouse
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 152 205 EM
Inhibitory and excitatory axon terminals share a common nano-architecture of their Cav2.1 (P/Q-type) Ca(2+) channels.
Althof D, Baehrens D, Watanabe M, Suzuki N, Fakler B, Kulik Á
Frontiers in cellular neuroscience (2015) 9: 315. 152 205 EM
Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).