Synaptic Systems - Ca2+ channel P/Q-type alpha-1A

Cat. No.: 152 205

Amount: 50 µg

Price: $465.00

Cat. No. 152 205	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet EM: yes gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P54282)
Reactivity	Reacts with: rat (P54282), mouse (P97445). Other species not tested yet.
Remarks	WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel P/Q-type alpha-1A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires mild fixation.
Data sheet	152_205.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 205

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 152 205 ICC; tested species: rat

Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 152 205 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 152 205 IHC; tested species: rat

Correct expression and localization of collagen xiii is crucial for the normal formation and function of the neuromuscular system.
Härönen H, Zainul Z, Naumenko N, Sormunen R, Miinalainen I, Shakirzyanova A, Santoleri S, Kemppainen AV, Giniatullin R, Pihlajaniemi T, Heikkinen A, et al.
The European journal of neuroscience (2019) : . 152 205 IHC; tested species: mouse

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 152 205 IHC; tested species: mouse

Developmental transformation of Ca2+ channel-vesicle nanotopography at a central GABAergic synapse.
Chen JJ, Kaufmann WA, Chen C, Arai I, Kim O, Shigemoto R, Jonas P
Neuron (2024) 1125: 755-771.e9. 152 205 EM; tested species: mouse

Nanoscale Phosphoinositide Distribution on Cell Membranes of Mouse Cerebellar Neurons.
Eguchi K, Le Monnier E, Shigemoto R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4323: 4197-4216. 152 205 EM; tested species: mouse

Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images.
Guerrero-Given D, Goldin SL, Thomas CI, Anthony SA, Jerez D, Kamasawa N
Frontiers in neuroanatomy (2022) 16: 855218. 152 205 EM; tested species: mouse

Different priming states of synaptic vesicles underlie distinct release probabilities at hippocampal excitatory synapses.
Aldahabi M, Balint F, Holderith N, Lorincz A, Reva M, Nusser Z
Neuron (2022) : . 152 205 EM; tested species: mouse

Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 152 205 EM; tested species: mouse

Presynaptic development is controlled by the core active zone proteins CAST/ELKS.
Radulovic T, Dong W, Goral RO, Thomas CI, Veeraraghavan P, Montesinos MS, Guerrero-Given D, Goff K, Lübbert M, Kamasawa N, Ohtsuka T, et al.
The Journal of physiology (2020) 59812: 2431-2452. 152 205 EM; tested species: mouse

CAST/ELKS Proteins Control Voltage-Gated Ca2+ Channel Density and Synaptic Release Probability at a Mammalian Central Synapse.
Dong W, Radulovic T, Goral RO, Thomas C, Suarez Montesinos M, Guerrero-Given D, Hagiwara A, Putzke T, Hida Y, Abe M, Sakimura K, et al.
Cell reports (2018) 242: 284-293.e6. 152 205 EM; tested species: mouse

CaV2.1 α1 Subunit Expression Regulates Presynaptic CaV2.1 Abundance and Synaptic Strength at a Central Synapse.
Lübbert M, Goral RO, Keine C, Thomas C, Guerrero-Given D, Putzke T, Satterfield R, Kamasawa N, Young SM
Neuron (2018) : . 152 205 EM; tested species: mouse

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 152 205 EM

Inhibitory and excitatory axon terminals share a common nano-architecture of their Cav2.1 (P/Q-type) Ca(2+) channels.
Althof D, Baehrens D, Watanabe M, Suzuki N, Fakler B, Kulik Á
Frontiers in cellular neuroscience (2015) 9: 315. 152 205 EM

Cat. No.: 152 205

Amount: 50 µg

Price: $465.00

Variability in the Munc13-1 content of excitatory release sites.
Karlocai MR, Heredi J, Benedek T, Holderith N, Lorincz A, Nusser Z
eLife (2021) 10: . 152 205 EM; tested species: mouse

Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells.
Kerti-Szigeti K, Nusser Z
eLife (2016) 5: . 152 205 EM

IHC

Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca²⁺ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).

1 ) Wu LG et al., J. Neurosci. (1999)
2 ) Westenbroek RE et al., J. Neurosci. (1998)
3 ) Sakurai T et al., J. Cell Biol. (1996)
4 ) Westenbroek RE et al., J. Neurosci. (1995)
5 ) Sakurai T et al., J. Biol. Chem. (1995)
6 ) Starr TV et al., Proc. Natl. Acad. Sci. U.S.A. (1991)

Protocols

IHC

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Ca2+ channel P/Q-type alpha-1A antibody - 152 205

References for Ca2+ channel P/Q-type alpha-1A - 152 205