Synaptic Systems - Ca2+ channel P/Q-type alpha-1A

Cat. No.: 152 103

Amount: 50 µg

Price: $375.00

Cat. No. 152 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (see remarks) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 5000 gallery IHC: yes (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 856 to 888 from mouse Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P97445)
Reactivity	Reacts with: rat (P54282), mouse (P97445). Other species not tested yet.
Specificity	K.O. validated PubMed: 22701595
Remarks	WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel P/Q-type alpha-1A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: Cat. no. 152 203, is recommended for IHC.
Data sheet	152_103.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 103

Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 152 103 WB; tested species: mouse

Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 152 103 WB; tested species: mouse

The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 152 103 WB

Delayed postnatal loss of P/Q-type calcium channels recapitulates the absence epilepsy, dyskinesia, and ataxia phenotypes of genomic Cacna1a mutations.
Mark MD, Maejima T, Kuckelsberg D, Yoo JW, Hyde RA, Shah V, Gutierrez D, Moreno RL, Kruse W, Noebels JL, Herlitze S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3111: 4311-26. 152 103 WB

Synaptic NMDA receptor signalling controls R-type calcium channel recruitment.
Feng Z, Glebov OO
The European journal of neuroscience (2021) : . 152 103 ICC; tested species: rat

Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.
Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences (2020) 106: . 152 103 ICC; tested species: human,mouse

Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 103 ICC; tested species: human

Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 152 103 ICC; tested species: rat

Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 152 103 ICC

Bicistronic CACNA1A Gene Expression in Neurons Derived from Spinocerebellar Ataxia Type 6 Patient-Induced Pluripotent Stem Cells.
Bavassano C, Eigentler A, Stanika R, Obermair GJ, Boesch S, Dechant G, Nat R
Stem cells and development (2017) 2622: 1612-1625. 152 103 ICC; tested species: human

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 152 103 ICC; tested species: rat

α2δ expression sets presynaptic calcium channel abundance and release probability.
Hoppa MB, Lana B, Margas W, Dolphin AC, Ryan TA
Nature (2012) 4867401: 122-5. 152 103 ICC

Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 152 103 ICC

Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons.
Obermair GJ, Schlick B, Di Biase V, Subramanyam P, Gebhart M, Baumgartner S, Flucher BE
The Journal of biological chemistry (2010) 2858: 5776-91. 152 103 ICC

P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 152 103 IHC, EM; tested species: mouse

Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.
Sargoy A, Sun X, Barnes S, Brecha NC
PloS one (2014) 91: e84507. 152 103 IHC; tested species: rat

Postnatal loss of P/Q-type channels confined to rhombic-lip-derived neurons alters synaptic transmission at the parallel fiber to purkinje cell synapse and replicates genomic Cacna1a mutation phenotype of ataxia and seizures in mice.
Maejima T, Wollenweber P, Teusner LU, Noebels JL, Herlitze S, Mark MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3312: 5162-74. 152 103 IHC

Calcium channels in rat horizontal cells regulate feedback inhibition of photoreceptors through an unconventional GABA- and pH-sensitive mechanism.
Liu X, Hirano AA, Sun X, Brecha NC, Barnes S
The Journal of physiology (2013) 59113: 3309-24. 152 103 IHC; tested species: rat

Active zone protein Bassoon co-localizes with presynaptic calcium channel, modifies channel function, and recovers from aging related loss by exercise.
Nishimune H, Numata T, Chen J, Aoki Y, Wang Y, Starr MP, Mori Y, Stanford JA
PloS one (2012) 76: e38029. 152 103 IHC; KO verified; tested species: mouse

Cat. No.: 152 103

Amount: 50 µg

Price: $375.00

Synaptic NMDA receptor signalling controls R-type calcium channel recruitment.
Feng Z, Glebov OO
The European journal of neuroscience (2021) : . 152 103 ICC; tested species: rat

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 152 103 ICC; tested species: rat

α2δ expression sets presynaptic calcium channel abundance and release probability.
Hoppa MB, Lana B, Margas W, Dolphin AC, Ryan TA
Nature (2012) 4867401: 122-5. 152 103 ICC

ICC

Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca²⁺ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).

1 ) Wu LG et al., J. Neurosci. (1999)
2 ) Westenbroek RE et al., J. Neurosci. (1998)
3 ) Sakurai T et al., J. Cell Biol. (1996)
4 ) Westenbroek RE et al., J. Neurosci. (1995)
5 ) Sakurai T et al., J. Biol. Chem. (1995)
6 ) Starr TV et al., Proc. Natl. Acad. Sci. U.S.A. (1991)

Protocols

ICC

IHC

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Ca2+ channel P/Q-type alpha-1A antibody - 152 103 K.O.

References for Ca2+ channel P/Q-type alpha-1A - 152 103