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Ca2+ channel P/Q-type alpha-1A antibody - 152 103 K.O.

Rabbit polyclonal purified antibody
Cat. No.: 152 103
Amount: 50 µg
Price: $375.00
Cat. No. 152 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 5000 gallery  
IHC: yes (see remarks)
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 856 to 888 from mouse Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P97445)
Reactivity Reacts with: rat (P54282), mouse (P97445).
Other species not tested yet.
Specificity K.O. validated PubMed: 22701595
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel P/Q-type alpha-1A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: Cat. no. 152 203, is recommended for IHC.

Data sheet 152_103.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 103

Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 152 103 WB; tested species: mouse
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 152 103 WB
Delayed postnatal loss of P/Q-type calcium channels recapitulates the absence epilepsy, dyskinesia, and ataxia phenotypes of genomic Cacna1a mutations.
Mark MD, Maejima T, Kuckelsberg D, Yoo JW, Hyde RA, Shah V, Gutierrez D, Moreno RL, Kruse W, Noebels JL, Herlitze S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3111: 4311-26. 152 103 WB
Synaptic NMDA receptor signalling controls R-type calcium channel recruitment.
Feng Z, Glebov OO
The European journal of neuroscience (2021) : . 152 103 ICC; tested species: rat
Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.
Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences (2020) 106: . 152 103 ICC; tested species: human,mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 103 ICC; tested species: human
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 152 103 ICC; tested species: rat
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 152 103 ICC
Bicistronic CACNA1A Gene Expression in Neurons Derived from Spinocerebellar Ataxia Type 6 Patient-Induced Pluripotent Stem Cells.
Bavassano C, Eigentler A, Stanika R, Obermair GJ, Boesch S, Dechant G, Nat R
Stem cells and development (2017) 2622: 1612-1625. 152 103 ICC; tested species: human
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 152 103 ICC; tested species: rat
α2δ expression sets presynaptic calcium channel abundance and release probability.
Hoppa MB, Lana B, Margas W, Dolphin AC, Ryan TA
Nature (2012) 4867401: 122-5. 152 103 ICC
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 152 103 ICC
Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons.
Obermair GJ, Schlick B, Di Biase V, Subramanyam P, Gebhart M, Baumgartner S, Flucher BE
The Journal of biological chemistry (2010) 2858: 5776-91. 152 103 ICC
P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 152 103 IHC, EM; tested species: mouse
Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.
Sargoy A, Sun X, Barnes S, Brecha NC
PloS one (2014) 91: e84507. 152 103 IHC; tested species: rat
Postnatal loss of P/Q-type channels confined to rhombic-lip-derived neurons alters synaptic transmission at the parallel fiber to purkinje cell synapse and replicates genomic Cacna1a mutation phenotype of ataxia and seizures in mice.
Maejima T, Wollenweber P, Teusner LU, Noebels JL, Herlitze S, Mark MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3312: 5162-74. 152 103 IHC
Calcium channels in rat horizontal cells regulate feedback inhibition of photoreceptors through an unconventional GABA- and pH-sensitive mechanism.
Liu X, Hirano AA, Sun X, Brecha NC, Barnes S
The Journal of physiology (2013) 59113: 3309-24. 152 103 IHC; tested species: rat
Active zone protein Bassoon co-localizes with presynaptic calcium channel, modifies channel function, and recovers from aging related loss by exercise.
Nishimune H, Numata T, Chen J, Aoki Y, Wang Y, Starr MP, Mori Y, Stanford JA
PloS one (2012) 76: e38029. 152 103 IHC; KO verified; tested species: mouse
P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 152 103 IHC, EM; tested species: mouse
Cat. No.: 152 103
Amount: 50 µg
Price: $375.00
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 152 103 WB; tested species: mouse
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 152 103 WB
Delayed postnatal loss of P/Q-type calcium channels recapitulates the absence epilepsy, dyskinesia, and ataxia phenotypes of genomic Cacna1a mutations.
Mark MD, Maejima T, Kuckelsberg D, Yoo JW, Hyde RA, Shah V, Gutierrez D, Moreno RL, Kruse W, Noebels JL, Herlitze S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3111: 4311-26. 152 103 WB
Synaptic NMDA receptor signalling controls R-type calcium channel recruitment.
Feng Z, Glebov OO
The European journal of neuroscience (2021) : . 152 103 ICC; tested species: rat
Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.
Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences (2020) 106: . 152 103 ICC; tested species: human,mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 103 ICC; tested species: human
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 152 103 ICC; tested species: rat
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 152 103 ICC
Bicistronic CACNA1A Gene Expression in Neurons Derived from Spinocerebellar Ataxia Type 6 Patient-Induced Pluripotent Stem Cells.
Bavassano C, Eigentler A, Stanika R, Obermair GJ, Boesch S, Dechant G, Nat R
Stem cells and development (2017) 2622: 1612-1625. 152 103 ICC; tested species: human
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 152 103 ICC; tested species: rat
α2δ expression sets presynaptic calcium channel abundance and release probability.
Hoppa MB, Lana B, Margas W, Dolphin AC, Ryan TA
Nature (2012) 4867401: 122-5. 152 103 ICC
Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 152 103 ICC
Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons.
Obermair GJ, Schlick B, Di Biase V, Subramanyam P, Gebhart M, Baumgartner S, Flucher BE
The Journal of biological chemistry (2010) 2858: 5776-91. 152 103 ICC
P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 152 103 IHC, EM; tested species: mouse
Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.
Sargoy A, Sun X, Barnes S, Brecha NC
PloS one (2014) 91: e84507. 152 103 IHC; tested species: rat
Postnatal loss of P/Q-type channels confined to rhombic-lip-derived neurons alters synaptic transmission at the parallel fiber to purkinje cell synapse and replicates genomic Cacna1a mutation phenotype of ataxia and seizures in mice.
Maejima T, Wollenweber P, Teusner LU, Noebels JL, Herlitze S, Mark MD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3312: 5162-74. 152 103 IHC
Calcium channels in rat horizontal cells regulate feedback inhibition of photoreceptors through an unconventional GABA- and pH-sensitive mechanism.
Liu X, Hirano AA, Sun X, Brecha NC, Barnes S
The Journal of physiology (2013) 59113: 3309-24. 152 103 IHC; tested species: rat
Active zone protein Bassoon co-localizes with presynaptic calcium channel, modifies channel function, and recovers from aging related loss by exercise.
Nishimune H, Numata T, Chen J, Aoki Y, Wang Y, Starr MP, Mori Y, Stanford JA
PloS one (2012) 76: e38029. 152 103 IHC; KO verified; tested species: mouse
P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 152 103 IHC, EM; tested species: mouse
Background

Voltage gated calcium channels (VGCCs), also referred to as voltage sensitive calcium channels (VSCCs), are present in most excitable cells. They mediate the influx of Ca2+ ions into the cell and trigger the release of neurotransmitters or hormons but are also involved in other calcium dependent processes like metabolism, cell proliferation and cell death.
VGCCs are composed of four subunits (α-1, α-2, β and δ) in a 1:1:1:1 ratio. The α-1A isoform occurs in VGCCs of the P/Q-type while isoform α-1B is found in the N-type. Both belong to the high voltage activated group (hva).