Tailor-made Antibodies
and Tools for Life Science

Ca2+ channel P/Q-type alpha-1A antibody - 152 103 K.O.

Rabbit polyclonal purified antibody
Cat. No.: 152 103
Amount: 50 µg
Price: $380.00
Cat. No. 152 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 5000 gallery  
IHC: yes (see remarks)
IHC-P: not tested yet
IHC-Fr: 1 : 500 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 856 to 888 from mouse Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P97445)
Reactivity Reacts with: rat (P54282), mouse (P97445).
Other species not tested yet.
Specificity K.O. validated PubMed: 22701595
Remarks

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. Ca2+ channel P/Q-type alpha-1A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC: Cat. no. 152 203 is recommended for IHC.
IHC-Fr: Formaldehyde or acetone fixation is recommended.

Data sheet 152_103.pdf

References for Ca2+ channel P/Q-type alpha-1A - 152 103

Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 152 103 WB; tested species: mouse
Rapid purification and metabolomic profiling of synaptic vesicles from mammalian brain.
Chantranupong L, Saulnier JL, Wang W, Jones DR, Pacold ME, Sabatini BL
eLife (2020) 9: . 152 103 WB; tested species: mouse
The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.
Liu C, Bickford LS, Held RG, Nyitrai H, Südhof TC, Kaeser PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3437: 12289-303. 152 103 WB
Delayed postnatal loss of P/Q-type calcium channels recapitulates the absence epilepsy, dyskinesia, and ataxia phenotypes of genomic Cacna1a mutations.
Mark MD, Maejima T, Kuckelsberg D, Yoo JW, Hyde RA, Shah V, Gutierrez D, Moreno RL, Kruse W, Noebels JL, Herlitze S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3111: 4311-26. 152 103 WB
Cat. No.: 152 103
Amount: 50 µg
Price: $380.00