Synaptic Systems

Cat. No.: 151 002

Amount: 200 µl

Price: $355.00

Cat. No. 151 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 662 to 769 from rat GRIP (UniProt Id: P97879)
Reactivity	Reacts with: human (Q9Y3R0), rat (P97879), mouse (Q925T6), hamster. Other species not tested yet.
Specificity	Recognizes GRIP 1 and GRIP 2.
Matching control protein/peptide	151-0P
Data sheet	151_002.pdf

References for GRIP - 151 002

Ethanol-induced AMPA alterations are mediated by mGLU5 receptors through miRNA upregulation in hippocampal slices.
Gerace E, Curti L, Caffino L, Bigagli E, Mottarlini F, Castillo Díaz F, Ilari A, Luceri C, Dani C, Fumagalli F, Masi A, et al.
European journal of pharmacology (2023) 955: 175878. 151 002 WB; tested species: rat

Hypersensitivity to amphetamine's psychomotor and reinforcing effects in serotonin transporter knockout rats: Glutamate in the nucleus accumbens.
Caffino L, Verheij MMM, Roversi K, Targa G, Mottarlini F, Popik P, Nikiforuk A, Golebiowska J, Fumagalli F, Homberg JR
British journal of pharmacology (2020) 17719: 4532-4547. 151 002 WB; tested species: rat

Astrocytes regulate GluR2 expression in motor neurons and their vulnerability to excitotoxicity.
Van Damme P, Bogaert E, Dewil M, Hersmus N, Kiraly D, Scheveneels W, Bockx I, Braeken D, Verpoorten N, Verhoeven K, Timmerman V, et al.
Proceedings of the National Academy of Sciences of the United States of America (2007) 10437: 14825-30. 151 002 WB

Cat. No.: 151 002

Amount: 200 µl

Price: $355.00

Background

The glutamate receptor interacting protein GRIP is a post-synaptic scaffolding protein consisting of seven PDZ domains. It has been shown to interact with a diverse array of proteins like several AMPA receptors, HAP1-A, liprin-α and LAR receptor protein tyrosine phosphatases (LAR-RPTPs).
GRIP is also involved in the synaptic targeting of AMPA receptors.

1 ) Stegmüller J et al., J. Biol. Chem. (2003)
2 ) Hirbec H et al., J. Biol. Chem. (2002)
3 ) Yu G et al., Brain Res. Dev. Brain Res. (2001)
4 ) Brückner K et al., Neuron (1999)
5 ) Dong H et al., Nature (1997)

Protocols

OTHER

Preabsorbtion

Other products for this target

GRIP antibody - 151 002

References for GRIP - 151 002