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Home > Products > 147 111BT

Gephyrin antibody - 147 111BT K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Mouse monoclonal purified IgG
Cat. No.: 147 111BT
Amount: 100 µg
Price: $475.00
Cat. No. 147 111BT 100 µg purified IgG, lyophilized, labeled with Biotin.
For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP-staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not recommended
IHC-P: not recommended
IHC-Fr: 1 : 500 (see remarks)
ELISA: yes
Label biotin
Clone 3B11
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 307 to 735 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 326 to 550 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), zebrafish.
Other species not tested yet.
Specificity Detects all splice variants that contain a complete E-domain including the C6 domain. K.O. validated
Remarks

This antibody is highly recommended for Western blot experiments and immunoprecipitation.
IHC-Fr: Fixation with PFA/formaldehyde or ice-cold methanol/acetone is recommended
Data sheet 147_111bt.pdf

References for Gephyrin - 147 111BT

Neurexin 1 variants as risk factors for suicide death.
William N, Reissner C, Sargent R, Darlington TM, DiBlasi E, Li QS, Keeshin B, Callor WB, Ferris E, Jerominski L, Smith KR, et al.
Molecular psychiatry (2021) : . 147 111BT ICC; tested species: human
Cat. No.: 147 111BT
Amount: 100 µg
Price: $475.00
WB
ICC
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