|Cat. No. 147 111BT||100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: yes , methanol-acetone fixation
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 307 to 735 from rat Gephyrin (UniProt Id: Q03555)|
Epitop: AA 326 to 550 from rat Gephyrin (UniProt Id: Q03555)
Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), zebrafish.
Other species not tested yet.
|Specificity||Detects all splice variants that contain a complete E-domain including the C6 domain. K.O.|
|Remarks||This antibody is highly recommended for Western blot experiments and immunoprecipitation.|
Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.