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Home > Products > 147 111BT

Gephyrin antibody - 147 111BT K.O.

Gephyrin is a scaffolding protein associated with inhibitory neurotransmitter receptors
Mouse monoclonal purified IgG
Cat. No.: 147 111BT
Amount: 100 µg
Price: $470.00
Cat. No. 147 111BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.
For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP-staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not recommended
IHC-P: not recommended
IHC-Fr: 1 : 500 (see remarks)
ELISA: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label biotin
Clone 3B11
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 307 to 735 from rat Gephyrin (UniProt Id: Q03555)
Epitop AA 326 to 550 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), zebrafish.
Other species not tested yet.
Specificity Detects all splice variants that contain a complete E-domain including the C6 domain. K.O. validated
Remarks

This antibody is highly recommended for Western blot experiments and immunoprecipitation.
IHC-Fr: Fixation with PFA/formaldehyde or ice-cold methanol/acetone is recommended
Data sheet 147_111bt.pdf

References for Gephyrin - 147 111BT

Neurexin 1 variants as risk factors for suicide death.
William N, Reissner C, Sargent R, Darlington TM, DiBlasi E, Li QS, Keeshin B, Callor WB, Ferris E, Jerominski L, Smith KR, et al.
Molecular psychiatry (2021) : . 147 111BT ICC; tested species: human
Activity-dependent death of transient Cajal-Retzius neurons is required for functional cortical wiring.
Riva M, Genescu I, Habermacher C, Orduz D, Ledonne F, Rijli FM, López-Bendito G, Coppola E, Garel S, Angulo MC, Pierani A, et al.
eLife (2019) 8: . 147 111BT IHC; tested species: mouse
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 147 111BT IHC; tested species: mouse
Cat. No.: 147 111BT
Amount: 100 µg
Price: $470.00
Neurexin 1 variants as risk factors for suicide death.
William N, Reissner C, Sargent R, Darlington TM, DiBlasi E, Li QS, Keeshin B, Callor WB, Ferris E, Jerominski L, Smith KR, et al.
Molecular psychiatry (2021) : . 147 111BT ICC; tested species: human
Activity-dependent death of transient Cajal-Retzius neurons is required for functional cortical wiring.
Riva M, Genescu I, Habermacher C, Orduz D, Ledonne F, Rijli FM, López-Bendito G, Coppola E, Garel S, Angulo MC, Pierani A, et al.
eLife (2019) 8: . 147 111BT IHC; tested species: mouse
IgSF9b regulates anxiety behaviors through effects on centromedial amygdala inhibitory synapses.
Babaev O, Cruces-Solis H, Piletti Chatain C, Hammer M, Wenger S, Ali H, Karalis N, de Hoz L, Schlüter OM, Yanagawa Y, Ehrenreich H, et al.
Nature communications (2018) 91: 5400. 147 111BT IHC; tested species: mouse
WB
ICC
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.
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