Home|||||||Technical Support|

Gephyrin - 147 011C3

A protein associated with inhibitory neurotransmitter receptors
Monoclonal mouse purified IgG
Cat. No.: 147 011C3
Amount: 100 µg
Price: 465.00 €
Cat. No. 147 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 250 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone mAb7a
Subtype IgG1 (κ light chain)
Immunogen Nativ Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant phosphorylated at Ser-270. K.O.
Data sheet 147_011c3.pdf

References for Gephyrin - 147 011C3

Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins.
Smith KR, Jones KA, Kopeikina KJ, Burette AC, Copits BA, Yoon S, Forrest MP, Fawcett-Patel JM, Hanley JG, Weinberg RJ, Swanson GT, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3746: 11127-11139. 147 011C3 ICC; tested species: rat
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 147 011C3 IHC; tested species: rat
The role of calretinin-expressing granule cells in olfactory bulb functions and odor behavior.
Hardy D, Malvaut S, Breton-Provencher V, Saghatelyan A
Scientific reports (2018) 81: 9385. 147 011C3 IHC; tested species: mouse
α5GABAA Receptors Mediate Tonic Inhibition in the Spinal Cord Dorsal Horn and Contribute to the Resolution Of Hyperalgesia.
Perez-Sanchez J, Lorenzo LE, Lecker I, Zurek AA, Labrakakis C, Bridgwater EM, Orser BA, De Koninck Y, Bonin RP
Journal of neuroscience research (2017) 956: 1307-1318. 147 011C3 IHC; tested species: mouse
Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.
Lorenzo LE, Godin AG, Wang F, St-Louis M, Carbonetto S, Wiseman PW, Ribeiro-da-Silva A, De Koninck Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8300-17. 147 011C3 IHC; tested species: rat
Cat. No.: 147 011C3
Quantity: 100 µg
Price: 465.00 €
Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins.
Smith KR, Jones KA, Kopeikina KJ, Burette AC, Copits BA, Yoon S, Forrest MP, Fawcett-Patel JM, Hanley JG, Weinberg RJ, Swanson GT, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3746: 11127-11139. 147 011C3 ICC; tested species: rat
Enhancing neuronal chloride extrusion rescues α2/α3 GABAA-mediated analgesia in neuropathic pain.
Lorenzo LE, Godin AG, Ferrini F, Bachand K, Plasencia-Fernandez I, Labrecque S, Girard AA, Boudreau D, Kianicka I, Gagnon M, Doyon N, et al.
Nature communications (2020) 111: 869. 147 011C3 IHC; tested species: rat
The role of calretinin-expressing granule cells in olfactory bulb functions and odor behavior.
Hardy D, Malvaut S, Breton-Provencher V, Saghatelyan A
Scientific reports (2018) 81: 9385. 147 011C3 IHC; tested species: mouse
α5GABAA Receptors Mediate Tonic Inhibition in the Spinal Cord Dorsal Horn and Contribute to the Resolution Of Hyperalgesia.
Perez-Sanchez J, Lorenzo LE, Lecker I, Zurek AA, Labrakakis C, Bridgwater EM, Orser BA, De Koninck Y, Bonin RP
Journal of neuroscience research (2017) 956: 1307-1318. 147 011C3 IHC; tested species: mouse
Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.
Lorenzo LE, Godin AG, Wang F, St-Louis M, Carbonetto S, Wiseman PW, Ribeiro-da-Silva A, De Koninck Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8300-17. 147 011C3 IHC; tested species: rat
Background

Gephyrin is a bifunctional protein which is essential for both synaptic clustering of inhibitory neurotransmitter receptors in the central nervous system and the biosynthesis of the molybdenum cofactor (MoCo) in peripheral tissues. It co-purifies with the inhibitory glycine receptor (GlyR) and is expressed abundantly in all brain areas which contain synapses.

Protocols