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Glycine receptor beta - 146 211

An inhibitory receptor in the nervous system
Monoclonal mouse purified IgG
Cat. No.: 146 211
Amount: 100 µg
Price: 415.00 €
Cat. No. 146 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: not recommended (see remarks)
IP: not tested yet
ICC: yes (methanol fixation)
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: not recommended
Clone 299E7
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 336 to 455 from rat Glycine receptor β (UniProt Id: P20781)
Epitop aa 336 - 355
Reactivity Reacts with: rat (P20781), mouse (P48168), monkey.
Other species not tested yet.
Specificity Specific for the glycine receptor β-subunit.
Remarks

WB: Detection of overexpressed protein is possible. Endogenous levels are hardly detectable.
IHC: Fixation for 10-15 min with cold 2 % PFA solution is recommended. Include 0,5 % triton X 100 in all blocking and antibody incubation steps.

Data sheet 146_211.pdf

References for Glycine receptor beta - 146 211

Disruption of a Structurally Important Extracellular Element in the Glycine Receptor Leads to Decreased Synaptic Integration and Signaling Resulting in Severe Startle Disease.
Schaefer N, Berger A, van Brederode J, Zheng F, Zhang Y, Leacock S, Littau L, Jablonka S, Malhotra S, Topf M, Winter F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7948-7961. 146 211 WB; tested species: mouse
Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody.
Weltzien F, Puller C, O'Sullivan GA, Paarmann I, Betz H
The Journal of comparative neurology (2012) 52017: 3962-81. 146 211 ICC, IHC
Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody.
Weltzien F, Puller C, O'Sullivan GA, Paarmann I, Betz H
The Journal of comparative neurology (2012) 52017: 3962-81. 146 211 ICC, IHC
Presence of ethanol-sensitive glycine receptors in medium spiny neurons in the mouse nucleus accumbens.
Förstera B, Muñoz B, Lobo MK, Chandra R, Lovinger DM, Aguayo LG
The Journal of physiology (2017) 59515: 5285-5300. 146 211 IHC-P; tested species: mouse
Cat. No.: 146 211
Quantity: 100 µg
Price: 415.00 €
Disruption of a Structurally Important Extracellular Element in the Glycine Receptor Leads to Decreased Synaptic Integration and Signaling Resulting in Severe Startle Disease.
Schaefer N, Berger A, van Brederode J, Zheng F, Zhang Y, Leacock S, Littau L, Jablonka S, Malhotra S, Topf M, Winter F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7948-7961. 146 211 WB; tested species: mouse
Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody.
Weltzien F, Puller C, O'Sullivan GA, Paarmann I, Betz H
The Journal of comparative neurology (2012) 52017: 3962-81. 146 211 ICC, IHC
Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody.
Weltzien F, Puller C, O'Sullivan GA, Paarmann I, Betz H
The Journal of comparative neurology (2012) 52017: 3962-81. 146 211 ICC, IHC
Presence of ethanol-sensitive glycine receptors in medium spiny neurons in the mouse nucleus accumbens.
Förstera B, Muñoz B, Lobo MK, Chandra R, Lovinger DM, Aguayo LG
The Journal of physiology (2017) 59515: 5285-5300. 146 211 IHC-P; tested species: mouse
Background
The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.