Glycine receptor alpha1 antibody - 146 113

An inhibitory receptor in the nervous system

Rabbit polyclonal purified antibody

Cat. No.: 146 113

Amount: 100 µl

Price: $380.00

Cat. No. 146 113	100 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P (FFPE): not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 29 to 50 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity	Reacts with: mouse (Q64018), rat (P07727). Other species not tested yet.
Data sheet	Datasheet 146_113

Cat. No.: 146 113

Amount: 100 µl

Price: $380.00

IHC

Background

The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.

1 ) Bechade C et al., Eur. J. Neurosci. (1996)
2 ) Becker CM et al., J. Neurosci. (1986)
3 ) Altschuler RA et al., Brain Res. (1986)
4 ) Triller A et al., J. Cell Biol. (1985)
5 ) Graham D et al., Biochemistry (1985)

Protocols

IHC

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