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Home > Products > 143 103

ERC2 antibody - 143 103 K.O.

ERCs are RIM binding proteins
Rabbit polyclonal purified antibody
Cat. No.: 143 103
Amount: 50 µg
Price: $375.00
Cat. No. 143 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 655 to 670 from rat ERC2 (UniProt Id: Q8K3M6)
Reactivity Reacts with: rat (Q8K3M6), mouse (Q6PH08), hamster.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 27422015
Matching control protein/peptide 143-1P
Data sheet 143_103.pdf

References for ERC2 - 143 103

The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 143 103 WB; tested species: rat
The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.
Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Fukaya M, Kitajima I, Takai Y, Watanabe M, et al.
The European journal of neuroscience (2016) 445: 2272-84. 143 103 WB; KO verified
Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 143 103 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 143 103 WB
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 103 WB, IHC
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 103 WB, IHC
Cat. No.: 143 103
Amount: 50 µg
Price: $375.00
The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons.
Lira M, Arancibia D, Orrego PR, Montenegro-Venegas C, Cruz Y, García J, Leal-Ortiz S, Godoy JA, Gundelfinger ED, Inestrosa NC, Garner CC, et al.
Molecular neurobiology (2018) : . 143 103 WB; tested species: rat
The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.
Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Fukaya M, Kitajima I, Takai Y, Watanabe M, et al.
The European journal of neuroscience (2016) 445: 2272-84. 143 103 WB; KO verified
Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 143 103 WB
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 143 103 WB
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 103 WB, IHC
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 103 WB, IHC
WB
Background

ELKS, also referred to as ERCs (ERC 1 and ERC 2) and CAST, are related proteins which share an identical C-terminal sequence. They interact with the conserved RIM PDZ domain via an unusual PDZ binding motif. Two splice variants of ERC 1 (1a and 1b) have been described. ERC 1b (CAST 2a) binds to RIM and is expressed exclusively in the brain. ERC 1a is a ubiquitously expressed cytosolic protein. ERC 2 (CAST 1) is only expressed as a single RIM binding variant.
All ERCs have been shown to interact with Rab 6, a protein involved in membrane trafficking at the Golgi complex. The function of these proteins has not been determined yet. They may link Rab 6 mediated non-neuronal membrane traffic at the Golgi complex to neuronal membrane traffic at the active zone executed via RIMs.
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