Synaptic Systems

Cat. No.: 143 003

Amount: 50 µg

Price: $375.00

Cat. No. 143 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: 1 : 100 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 939 to 948 from rat Erc1b (UniProt Id: Q811U3-1)
Reactivity	Reacts with: human (Q8IUD2, O15083), rat (Q811U3, Q8K3M6), mouse (Q99MI1, Q6PH08), hamster. No signal: zebrafish. Other species not tested yet.
Specificity	Specific for the brain specific isoforms ERC 1b and ERC 2.
Matching control protein/peptide	143-0P
Data sheet	143_003.pdf

References for ERC1b/2 - 143 003

Liprin-α proteins are master regulators of human presynapse assembly.
Marcó de la Cruz B, Campos J, Molinaro A, Xie X, Jin G, Wei Z, Acuna C, Sterky FH
Nature neuroscience (2024) : . 143 003 WB, ICC; tested species: human

A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 143 003 WB, ICC; tested species: mouse

Syndapin I Loss-of-Function in Mice Leads to Schizophrenia-Like Symptoms.
Koch N, Koch D, Krueger S, Tröger J, Sabanov V, Ahmed T, McMillan LE, Wolf D, Montag D, Kessels MM, Balschun D, et al.
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 143 003 WB; tested species: mouse

α2δ-4 is required for the molecular and structural organization of rod and cone photoreceptor synapses.
Kerov V, Laird JG, Joiner ML, Knecht S, Soh D, Hagen J, Gardner SH, Gutierrez W, Yoshimatsu T, Bhattarai S, Puthussery T, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 143 003 WB; tested species: mouse

The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.
Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Fukaya M, Kitajima I, Takai Y, Watanabe M, et al.
The European journal of neuroscience (2016) 445: 2272-84. 143 003 WB

RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 143 003 WB; tested species: mouse

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC

RIM1α SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports (2013) 55: 1294-301. 143 003 WB; tested species: rat

Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission.
Spangler SA, Schmitz SK, Kevenaar JT, de Graaff E, de Wit H, Demmers J, Toonen RF, Hoogenraad CC
The Journal of cell biology (2013) 2016: 915-28. 143 003 ICC, WB; tested species: rat

Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 143 003 WB

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 143 003 WB; tested species: mouse

mSYD1A, a mammalian synapse-defective-1 protein, regulates synaptogenic signaling and vesicle docking.
Wentzel C, Sommer JE, Nair R, Stiefvater A, Sibarita JB, Scheiffele P
Neuron (2013) 786: 1012-23. 143 003 WB; tested species: mouse

Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 143 003 WB

Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing.
Lazarevic V, Schöne C, Heine M, Gundelfinger ED, Fejtova A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3128: 10189-200. 143 003 WB, ICC

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 143 003 WB

Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 143 003 WB, IHC

Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 143 003 ICC; tested species: rat

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC

Lateral mobility of presynaptic α7-containing nicotinic receptors and its relevance for glutamate release.
Gomez-Varela D, Berg DK
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3343: 17062-71. 143 003 ICC; tested species: rat

Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.
Vatan T, Minehart JA, Zhang C, Agarwal V, Yang J, Speer CM
STAR protocols (2021) 24: 100971. 143 003 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 143 003 IHC; tested species: rat

ELKS1 and Ca(2+) channel subunit β4 interact and colocalize at cerebellar synapses.
Billings SE, Clarke GL, Nishimune H
Neuroreport (2012) 231: 49-54. 143 003 IHC

Active zone proteins are dynamically associated with synaptic ribbons in rat pinealocytes.
Spiwoks-Becker I, Maus C, tom Dieck S, Fejtová A, Engel L, Wolloscheck T, Wolfrum U, Vollrath L, Spessert R
Cell and tissue research (2008) 3332: 185-95. 143 003 IHC; tested species: rat

Cat. No.: 143 003

Amount: 50 µg

Price: $375.00

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC

RIM1α SUMOylation is required for fast synaptic vesicle exocytosis.
Girach F, Craig TJ, Rocca DL, Henley JM
Cell reports (2013) 55: 1294-301. 143 003 WB; tested species: rat

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 143 003 WB; tested species: mouse

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 143 003 WB, ICC

ELKS1 and Ca(2+) channel subunit β4 interact and colocalize at cerebellar synapses.
Billings SE, Clarke GL, Nishimune H
Neuroreport (2012) 231: 49-54. 143 003 IHC

ICC

IHC

Background

ELKS, also referred to as ERCs (ERC 1 and ERC 2) and CAST, are related proteins which share an identical C-terminal sequence. They interact with the conserved RIM PDZ domain via an unusual PDZ binding motif. Two splice variants of ERC 1 (1a and 1b) have been described. ERC 1b (CAST 2a) binds to RIM and is expressed exclusively in the brain. ERC 1a is a ubiquitously expressed cytosolic protein. ERC 2 (CAST 1) is only expressed as a single RIM binding variant.
All ERCs have been shown to interact with Rab 6, a protein involved in membrane trafficking at the Golgi complex. The function of these proteins has not been determined yet. They may link Rab 6 mediated non-neuronal membrane traffic at the Golgi complex to neuronal membrane traffic at the active zone executed via RIMs.

1 ) Takao-Rikitsu E et al., J. Cell Biol. (2004)
2 ) Deguchi-Tawarada M et al., Genes Cells (2004)
3 ) Ko J et al., J. Biol. Chem. (2003)
4 ) Ohtsuka T et al., J. Cell Biol. (2002)
5 ) Wang Y et al., Proc. Natl. Acad. Sci. U.S.A. (2002)

Protocols

Standard protocol

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

ERC1b/2 antibody - 143 003

References for ERC1b/2 - 143 003