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Home > Products > 142 002

Piccolo antibody - 142 002 K.O.

Piccolo is a large multi-domain protein of the presynaptic active zone
Rabbit polyclonal antiserum
Cat. No.: 142 002
Amount: 200 µl
Price: $360.00
Cat. No. 142 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (ECL detection) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 4439 to 4776 from rat Piccolo (UniProt Id: Q9JKS6)
Reactivity Reacts with: rat (Q9JKS6), mouse (Q9QYX7).
Other species not tested yet.
Specificity K.O. validated PubMed: 27537483
Matching control protein/peptide 142-0P
Remarks

WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

This antibody detects an additional band of ~65 kDa.
Data sheet 142_002.pdf

References for Piccolo - 142 002

ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, et al.
Human molecular genetics (2021) : . 142 002 WB, IP; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 142 002 WB; KO verified; tested species: rat
Presynaptic calcium channels and α3-integrins are complexed with synaptic cleft laminins, cytoskeletal elements and active zone components.
Carlson SS, Valdez G, Sanes JR
Journal of neurochemistry (2010) 1153: 654-66. 142 002 WB; tested species: mouse
APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.
Szodorai A, Kuan YH, Hunzelmann S, Engel U, Sakane A, Sasaki T, Takai Y, Kirsch J, Müller U, Beyreuther K, Brady S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 2946: 14534-44. 142 002 WB
Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 142 002 WB, IHC
Cat. No.: 142 002
Amount: 200 µl
Price: $360.00
WB
ICC
IHC
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