Synaptic Systems

Cat. No.: 141 016

Amount: 200 µl

Price: $370.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 141 016	200 µl antibody, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues corresponding to central region rat Bassoon. (UniProt Id: O88778)
Reactivity	Reacts with: rat (O88778), mouse (O88737). Other species not tested yet.
Specificity	Specific for bassoon.
Data sheet	141_016.pdf

References for Bassoon - 141 016

Development and Optimization of a Multilayer Rat Purkinje Neuron Culture.
Uggerud IM, Kråkenes T, Hirai H, Vedeler CA, Schubert M
Cerebellum (London, England) (2023) : . 141 016 ICC; tested species: rat

Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 141 016 ICC; tested species: mouse

A genetic variant of the Wnt receptor LRP6 accelerates synapse degeneration during aging and in Alzheimer's disease.
Jones ME, Büchler J, Dufor T, Palomer E, Teo S, Martin-Flores N, Boroviak K, Metzakopian E, Gibb A, Salinas PC
Science advances (2023) 92: eabo7421. 141 016 ICC, IHC; tested species: mouse

Interaction between Teneurin-2 and microtubules via EB proteins provides a platform for GABAA receptor exocytosis.
Ichinose S, Susuki Y, Hosoi N, Kaneko R, Ebihara M, Hirai H, Iwasaki H
eLife (2023) 12: . 141 016 ICC; tested species: mouse

S-SCAM is essential for synapse formation.
Wittenmayer N, Petkova-Tuffy A, Borgmeyer M, Lee C, Becker J, Böning A, Kügler S, Rhee J, Viotti JS, Dresbach T
Frontiers in cellular neuroscience (2023) 17: 1182493. 141 016 ICC; tested species: rat

Ribosomal RACK1 Regulates the Dendritic Arborization by Repressing FMRP Activity.
Romano N, Di Giacomo B, Nobile V, Borreca A, Willems D, Tilesi F, Catalani E, Agrawal M, Welshhans K, Ricciardi S, Cervia D, et al.
International journal of molecular sciences (2022) 2319: . 141 016 ICC; tested species: mouse

Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher M, Niebling S, Han Y, Molodenskiy D, Hassani Nia F, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M, et al.
eLife (2021) 10: . 141 016 ICC; tested species: rat

Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer's disease, restores synapse integrity and memory in a disease mouse model.
Martin Flores N, Podpolny M, McLeod F, Workman I, Crawford K, Ivanov D, Leonenko G, Escott-Price V, Salinas PC
eLife (2024) 12: . 141 016 IHC; tested species: mouse

Mechanisms of simultaneous linear and nonlinear computations at the mammalian cone photoreceptor synapse.
Grabner CP, Futagi D, Shi J, Bindokas V, Kitano K, Schwartz EA, DeVries SH
Nature communications (2023) 141: 3486. 141 016 IHC

Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing.
Michanski S, Kapoor R, Steyer AM, Möbius W, Früholz I, Ackermann F, Gültas M, Garner CC, Hamra FK, Neef J, Strenzke N, et al.
EMBO reports (2023) 249: e56702. 141 016 IHC; tested species: rat

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).
Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW
eLife (2022) 11: . 141 016 IHC; tested species: mouse

Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 141 016 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 141 016 IHC; tested species: rat

Coherent Activity between the Prelimbic and Auditory Cortex in the Slow-Gamma Band Underlies Fear Discrimination.
Concina G, Cambiaghi M, Renna A, Sacchetti B
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3839: 8313-8328. 141 016 IHC; tested species: rat

Cat. No.: 141 016

Amount: 200 µl

Price: $370.00

ICC

IHC

IHC-P

Background

Bassoon is a large protein which consists of an N-terminal Zn²⁺ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn²⁺ domain but the potential partners have not been determined yet.

1 ) Dresbach T et al., Mol. Cell. Neurosci. (2003)
2 ) Shapira M et al., Neuron (2003)
3 ) Altrock WD et al., Neuron (2003)
4 ) Dick O et al., Neuron (2003)
5 ) Dick O et al., J. Comp. Neurol. (2001)
6 ) Sanmartí-Vila L et al., Biochem. Biophys. Res. Commun. (2000)
7 ) tom Dieck S et al., J. Cell Biol. (1998)

Protocols

ICC

IHC

IHC-P

Other products for this target

Bassoon antibody - 141 016

References for Bassoon - 141 016