Tailor-made Antibodies
and Tools for Life Science

Bassoon antibody - 141 013

Bassoon is a large multi-domain protein of the presynaptic active zone
Rabbit polyclonal purified antibody
Cat. No.: 141 013
Amount: 50 µg
Price: $380.00
Cat. No. 141 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to residues corresponding to central region rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for bassoon.
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 141_013.pdf

References for Bassoon - 141 013

Pre- and postsynaptic nanostructures increase in size and complexity after induction of long-term potentiation.
Clavet-Fournier V, Lee C, Wegner W, Brose N, Rhee J, Willig KI
iScience (2024) 271: 108679. 141 013 ICC; tested species: rat
Synaptic and functional alterations in the development of mutant huntingtin expressing hiPSC-derived neurons.
Dinamarca MC, Colombo L, Tousiaki NE, Müller M, Pecho-Vrieseling E
Frontiers in molecular biosciences (2022) 9: 916019. 141 013 ICC; tested species: human
Cat. No.: 141 013
Amount: 50 µg
Price: $380.00