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Bassoon - 141 013

A large multi-domain protein of the presynaptic active zone
Polyclonal rabbit purified antibody
Cat. No.: 141 013
Amount: 50 µg
Price: $370.00
Cat. No. 141 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 756 to 1001 from rat Bassoon (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for bassoon.
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 141_013.pdf

References for Bassoon - 141 013

Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 141 013 IHC; tested species: mouse
Cat. No.: 141 013
Quantity: 50 µg
Price: $370.00
Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 141 013 IHC; tested species: mouse
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.