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Bassoon - 141 011

A large multi-domain protein of the presynaptic active zone
Monoclonal mouse purified IgG
Cat. No.: 141 011
Amount: 100 µg
Price: 415.00 €
Cat. No. 141 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not recommended
IHC-P/FFPE: 1 : 500 gallery  
ELISA: yes (see remarks)
Clone 219E1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)
Epitop Epitop: AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for bassoon.
Matching control protein/peptide 141-0P
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 141 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 141_011.pdf

References for Bassoon - 141 011

Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Rapid 3D Enhanced Resolution Microscopy Reveals Diversity in Dendritic Spinule Dynamics, Regulation, and Function.
Zaccard CR, Shapiro L, Martin-de-Saavedra MD, Pratt C, Myczek K, Song A, Forrest MP, Penzes P
Neuron (2020) : . 141 011 ICC; tested species: mouse
eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology.
Bettegazzi B, Bellani S, Roncon P, Guarnieri FC, Bertero A, Codazzi F, Valtorta F, Simonato M, Grohovaz F, Zacchetti D
Scientific reports (2017) 71: 10563. 141 011 ICC; tested species: rat
Remodelling of spared proprioceptive circuit involving a small number of neurons supports functional recovery.
Hollis ER, Ishiko N, Pessian M, Tolentino K, Lee-Kubli CA, Calcutt NA, Zou Y
Nature communications (2015) 6: 6079. 141 011 IHC
Cat. No.: 141 011
Quantity: 100 µg
Price: 415.00 €
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Rapid 3D Enhanced Resolution Microscopy Reveals Diversity in Dendritic Spinule Dynamics, Regulation, and Function.
Zaccard CR, Shapiro L, Martin-de-Saavedra MD, Pratt C, Myczek K, Song A, Forrest MP, Penzes P
Neuron (2020) : . 141 011 ICC; tested species: mouse
eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology.
Bettegazzi B, Bellani S, Roncon P, Guarnieri FC, Bertero A, Codazzi F, Valtorta F, Simonato M, Grohovaz F, Zacchetti D
Scientific reports (2017) 71: 10563. 141 011 ICC; tested species: rat
Remodelling of spared proprioceptive circuit involving a small number of neurons supports functional recovery.
Hollis ER, Ishiko N, Pessian M, Tolentino K, Lee-Kubli CA, Calcutt NA, Zou Y
Nature communications (2015) 6: 6079. 141 011 IHC
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.