Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 141 011

Bassoon antibody - 141 011

Bassoon is a large multi-domain protein of the presynaptic active zone
Mouse monoclonal purified IgG
Cat. No.: 141 011
Amount: 100 µg
Price: $415.00
Cat. No. 141 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not recommended
IHC-P: 1 : 500 gallery  
ELISA: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone 219E1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for Bassoon
Matching control protein/peptide 141-0P
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 141 003 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 141_011.pdf

References for Bassoon - 141 011

Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Real-time visualization of structural dynamics of synapses in live cells in vivo.
Son S, Nagahama K, Lee J, Jung K, Kwak C, Kim J, Noh YW, Kim E, Lee S, Kwon HB, Heo WD, et al.
Nature methods (2024) : . 141 011 ICC; tested species: rat
Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis.
Ogunmowo TH, Jing H, Raychaudhuri S, Kusick GF, Imoto Y, Li S, Itoh K, Ma Y, Jafri H, Dalva MB, Chapman ER, et al.
Nature communications (2023) 141: 2888. 141 011 ICC; tested species: mouse
Plasticity-Related Gene 5 Is Expressed in a Late Phase of Neurodifferentiation After Neuronal Cell-Fate Determination.
Gross I, Brandt N, Vonk D, Köper F, Wöhlbrand L, Rabus R, Witt M, Heep A, Plösch T, Hipp MS, Bräuer AU, et al.
Frontiers in cellular neuroscience (2022) 16: 797588. 141 011 ICC; tested species: mouse
Dynamin is primed at endocytic sites for ultrafast endocytosis.
Imoto Y, Raychaudhuri S, Ma Y, Fenske P, Sandoval E, Itoh K, Blumrich EM, Matsubayashi HT, Mamer L, Zarebidaki F, Söhl-Kielczynski B, et al.
Neuron (2022) 11017: 2815-2835.e13. 141 011 ICC; tested species: mouse
A short isoform of STIM1 confers frequency-dependent synaptic enhancement.
Ramesh G, Jarzembowski L, Schwarz Y, Poth V, Konrad M, Knapp ML, Schwär G, Lauer AA, Grimm MOW, Alansary D, Bruns D, et al.
Cell reports (2021) 3411: 108844. 141 011 ICC; tested species: mouse
Rapid 3D Enhanced Resolution Microscopy Reveals Diversity in Dendritic Spinule Dynamics, Regulation, and Function.
Zaccard CR, Shapiro L, Martin-de-Saavedra MD, Pratt C, Myczek K, Song A, Forrest MP, Penzes P
Neuron (2020) : . 141 011 ICC; tested species: mouse
eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology.
Bettegazzi B, Bellani S, Roncon P, Guarnieri FC, Bertero A, Codazzi F, Valtorta F, Simonato M, Grohovaz F, Zacchetti D
Scientific reports (2017) 71: 10563. 141 011 ICC; tested species: rat
Neurexins cluster Ca2+ channels within the presynaptic active zone.
Luo F, Sclip A, Jiang M, Südhof TC
The EMBO journal (2020) 397: e103208. 141 011 IHC; tested species: mouse
Remodelling of spared proprioceptive circuit involving a small number of neurons supports functional recovery.
Hollis ER, Ishiko N, Pessian M, Tolentino K, Lee-Kubli CA, Calcutt NA, Zou Y
Nature communications (2015) 6: 6079. 141 011 IHC
Cat. No.: 141 011
Amount: 100 µg
Price: $415.00
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Real-time visualization of structural dynamics of synapses in live cells in vivo.
Son S, Nagahama K, Lee J, Jung K, Kwak C, Kim J, Noh YW, Kim E, Lee S, Kwon HB, Heo WD, et al.
Nature methods (2024) : . 141 011 ICC; tested species: rat
Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis.
Ogunmowo TH, Jing H, Raychaudhuri S, Kusick GF, Imoto Y, Li S, Itoh K, Ma Y, Jafri H, Dalva MB, Chapman ER, et al.
Nature communications (2023) 141: 2888. 141 011 ICC; tested species: mouse
Plasticity-Related Gene 5 Is Expressed in a Late Phase of Neurodifferentiation After Neuronal Cell-Fate Determination.
Gross I, Brandt N, Vonk D, Köper F, Wöhlbrand L, Rabus R, Witt M, Heep A, Plösch T, Hipp MS, Bräuer AU, et al.
Frontiers in cellular neuroscience (2022) 16: 797588. 141 011 ICC; tested species: mouse
Dynamin is primed at endocytic sites for ultrafast endocytosis.
Imoto Y, Raychaudhuri S, Ma Y, Fenske P, Sandoval E, Itoh K, Blumrich EM, Matsubayashi HT, Mamer L, Zarebidaki F, Söhl-Kielczynski B, et al.
Neuron (2022) 11017: 2815-2835.e13. 141 011 ICC; tested species: mouse
A short isoform of STIM1 confers frequency-dependent synaptic enhancement.
Ramesh G, Jarzembowski L, Schwarz Y, Poth V, Konrad M, Knapp ML, Schwär G, Lauer AA, Grimm MOW, Alansary D, Bruns D, et al.
Cell reports (2021) 3411: 108844. 141 011 ICC; tested species: mouse
Rapid 3D Enhanced Resolution Microscopy Reveals Diversity in Dendritic Spinule Dynamics, Regulation, and Function.
Zaccard CR, Shapiro L, Martin-de-Saavedra MD, Pratt C, Myczek K, Song A, Forrest MP, Penzes P
Neuron (2020) : . 141 011 ICC; tested species: mouse
eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology.
Bettegazzi B, Bellani S, Roncon P, Guarnieri FC, Bertero A, Codazzi F, Valtorta F, Simonato M, Grohovaz F, Zacchetti D
Scientific reports (2017) 71: 10563. 141 011 ICC; tested species: rat
Neurexins cluster Ca2+ channels within the presynaptic active zone.
Luo F, Sclip A, Jiang M, Südhof TC
The EMBO journal (2020) 397: e103208. 141 011 IHC; tested species: mouse
Remodelling of spared proprioceptive circuit involving a small number of neurons supports functional recovery.
Hollis ER, Ishiko N, Pessian M, Tolentino K, Lee-Kubli CA, Calcutt NA, Zou Y
Nature communications (2015) 6: 6079. 141 011 IHC
WB
ICC
IHC-P
Background

Bassoon is a large protein which consists of an N-terminal Zn2+ finger and several piccolo-bassoon homology domains (PBH-domains). It is generally found together with piccolo, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Bassoon was suggested to be a scaffolding element of the presynapse but deletion experiments in mice have shown that bassoon is also involved in synaptic vesicle cycling. Probably bassoon interacts with other protein factors via its Zn2+ domain but the potential partners have not been determined yet.
Other products for this target