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Home > Products > 141 011

Bassoon antibody - 141 011

Bassoon is a large multi-domain protein of the presynaptic active zone
Mouse monoclonal purified IgG
Cat. No.: 141 011
Amount: 100 µg
Price: $420.00
Cat. No. 141 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not recommended
IHC-P: 1 : 500 gallery  
ELISA: yes
Clone 219E1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat Bassoon. (UniProt Id: O88778)
Reactivity Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
Specificity Specific for Bassoon
Matching control protein/peptide 141-0P
Remarks

WB: Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE.
ICC: 2% formaldehyde/PFA fixation is recommended.
ELISA: The ELISA-protocol for membrane proteins is required.
Suitable as capture antibody for sandwich-ELISA. Please refer to the protocol for suitable detector antibodies.
Data sheet 141_011.pdf

References for Bassoon - 141 011

Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Cat. No.: 141 011
Amount: 100 µg
Price: $420.00
WB
ICC
IHC-P
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