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RIM 1 - 140 111

A putative Rab 3 effector protein of the active zone
Monoclonal mouse purified IgG
Cat. No.: 140 111
Amount: 100 µg
Price: 415.00 €
Cat. No. 140 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not recommended
IHC-P/FFPE: not recommended
Clone 360H6E4
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 207 to 366 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity Reacts with: rat (Q9JIR4).
Other species not tested yet.
Specificity Specific for RIM 1, no cross reactivity to RIM 2.
Remarks

WB: less sensitive compared to the rabbit polyclonal antibody 140 013.

Data sheet 140_111.pdf
Cat. No.: 140 111
Quantity: 100 µg
Price: 415.00 €
Background

RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.