|Cat. No. 140 111||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 500 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: not recommended
IHC-P/FFPE: not recommended
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 207 to 366 from rat Rim1 (UniProt Id: Q9JIR4)|
Reacts with: rat (Q9JIR4).
Other species not tested yet.
|Specificity||Specific for RIM 1, no cross reactivity to RIM 2.|
WB: less sensitive compared to the rabbit polyclonal antibody 140 013.
RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.