RIM1 antibody - 140 111

RIMs are Rab3 effector proteins of the cytomatrix at the active zone

Mouse monoclonal purified IgG

Cat. No.: 140 111

Amount: 100 µg

Price: $420.00

Cat. No. 140 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not recommended IHC-P (FFPE): not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	360H6E4
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 207 to 366 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity	Reacts with: rat (Q9JIR4). Other species not tested yet.
Remarks	WB: less sensitive compared to the rabbit polyclonal antibody 140 013.
Data sheet	Datasheet 140_111

Cat. No.: 140 111

Amount: 100 µg

Price: $420.00

ICC

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.

1 ) Wang Y et al., Genomics (2003)
2 ) Castillo PE et al., Nature (2002)
3 ) Schoch S et al., Nature (2002)
4 ) Wang Y et al., J. Biol. Chem. (2000)
5 ) Wang Y et al., Nature (1997)

Protocols

ICC

Fixed Cells

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