Tailor-made Antibodies
and Tools for Life Science

RIM2 antibody PDZ domain - 140 103

RIMs are Rab3 effector proteins of the cytomatrix at the active zone
Rabbit polyclonal purified antibody
Cat. No.: 140 103
Amount: 50 µg
Price: $380.00
Cat. No. 140 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 461 to 987 from rat Rim2 (UniProt Id: Q9JIS1)
Reactivity Reacts with: rat (Q9JIS1), mouse (Q9EQZ7), hamster, chicken, zebrafish.
Other species not tested yet.
Specificity RIM2 including splice variants, weak cross reactivity to RIM 1.
Remarks

ICC: This antibody works with PFA and methanol fixation.

Data sheet 140_103.pdf

References for RIM2 - 140 103

Glutamatergic synapses from the insular cortex to the basolateral amygdala encode observational pain.
Zhang MM, Geng AQ, Chen K, Wang J, Wang P, Qiu XT, Gu JX, Fan HW, Zhu DY, Yang SM, Chen QY, et al.
Neuron (2022) 11012: 1993-2008.e6. 140 103 WB; tested species: mouse
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.
Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, Imig C, et al.
Neuron (2021) : . 140 103 WB; tested species: mouse
ELKS/Voltage-Dependent Ca2+ Channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β Cells.
Ohara-Imaizumi M, Aoyagi K, Yamauchi H, Yoshida M, Mori MX, Hida Y, Tran HN, Ohkura M, Abe M, Akimoto Y, Nakamichi Y, et al.
Cell reports (2019) 265: 1213-1226.e7. 140 103 WB; tested species: mouse
Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 103 WB, IHC; tested species: mouse
Inter-channel scaffolding of presynaptic CaV2.2 via the C terminal PDZ ligand domain.
Gardezi SR, Li Q, Stanley EF
Biology open (2013) 25: 492-8. 140 103 WB
Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site.
Limbach C, Laue MM, Wang X, Hu B, Thiede N, Hultqvist G, Kilimann MW
Proceedings of the National Academy of Sciences of the United States of America (2011) 10831: E392-401. 140 103 WB
Molecular anatomy of the hair cell's ribbon synapse.
Uthaiah RC, Hudspeth AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3037: 12387-99. 140 103 WB
Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cells.
Kwan EP, Xie L, Sheu L, Ohtsuka T, Gaisano HY
Diabetes (2007) 5610: 2579-88. 140 103 WB
A proteomic screen for presynaptic terminal N-type calcium channel (CaV2.2) binding partners.
Khanna R, Zougman A, Stanley EF
Journal of biochemistry and molecular biology (2007) 403: 302-14. 140 103 WB
Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification.
Juranek J, Mukherjee K, Rickmann M, Martens H, Calka J, Südhof TC, Jahn R
The European journal of neuroscience (2006) 2411: 3043-52. 140 103 WB, IHC
ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells.
Inoue E, Deguchi-Tawarada M, Takao-Rikitsu E, Inoue M, Kitajima I, Ohtsuka T, Takai Y
Genes to cells : devoted to molecular & cellular mechanisms (2006) 116: 659-72. 140 103 WB, ICC
ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence microscopy.
Ohara-Imaizumi M, Ohtsuka T, Matsushima S, Akimoto Y, Nishiwaki C, Nakamichi Y, Kikuta T, Nagai S, Kawakami H, Watanabe T, Nagamatsu S, et al.
Molecular biology of the cell (2005) 167: 3289-300. 140 103 WB
Molecular dissection of the photoreceptor ribbon synapse: physical interaction of Bassoon and RIBEYE is essential for the assembly of the ribbon complex.
tom Dieck S, Altrock WD, Kessels MM, Qualmann B, Regus H, Brauner D, Fejtová A, Bracko O, Gundelfinger ED, Brandstätter JH
The Journal of cell biology (2005) 1685: 825-36. 140 103 WB, IHC
Cat. No.: 140 103
Amount: 50 µg
Price: $380.00