Synaptic Systems

Cat. No.: 138 302

Amount: 200 µl

Price: $355.00

Cat. No. 138 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 1000 (see remarks) gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 496 to 515 from rat VMaT2 (UniProt Id: Q01827)
Reactivity	Reacts with: rat (Q8BRU6). Other species not tested yet.
Remarks	WB: VMaT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: Requires brief fixation with 4 % PFA for 2 min and subsequent ice cold methanol fixation for 10 min.
Data sheet	138_302.pdf

References for VMaT2 - 138 302

Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 138 302 WB; tested species: rat

Effects of sleep disruption on stress, nigrostriatal markers, and behavior in a chronic/progressive MPTP male mouse model of parkinsonism.
Xu M, Bohlen JK, Moore C, Nipper MA, Finn DA, Jones CE, Lim MM, Meshul CK
Journal of neuroscience research (2019) 9712: 1706-1719. 138 302 WB; tested species: mouse

Gender-dependent and genotype-sensitive monoaminergic changes induced by polychlorinated biphenyl 153 in the rat brain.
Dervola KS, Johansen EB, Walaas SI, Fonnum F
Neurotoxicology (2015) 50: 38-45. 138 302 WB

Amphetamine regulates NR2B expression in Go2α knockout mice and thereby sustains behavioral sensitization.
Brunk I, Sanchis-Segura C, Blex C, Perreau-Lenz S, Bilbao A, Spanagel R, Ahnert-Hilger G
Journal of neurochemistry (2010) 1151: 234-46. 138 302 WB; tested species: mouse

Chronic low-level lead exposure affects the monoaminergic system in the mouse superior olivary complex.
Fortune T, Lurie DI
The Journal of comparative neurology (2009) 5135: 542-58. 138 302 IHC, WB; tested species: mouse

Deletion of Go2alpha abolishes cocaine-induced behavioral sensitization by disturbing the striatal dopamine system.
Brunk I, Blex C, Sanchis-Segura C, Sternberg J, Perreau-Lenz S, Bilbao A, Hörtnagl H, Baron J, Juranek J, Laube G, Birnbaumer L, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2008) 2210: 3736-46. 138 302 WB

The first luminal domain of vesicular monoamine transporters mediates G-protein-dependent regulation of transmitter uptake.
Brunk I, Blex C, Rachakonda S, Höltje M, Winter S, Pahner I, Walther DJ, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28144: 33373-85. 138 302 WB, ICC

On-Site Formation of Functional Dopaminergic Presynaptic Terminals on Neuroligin-2-Modified Gold-Coated Microspheres.
Cho W, Jung M, Yoon SH, Jeon J, Oh MA, Kim JY, Park M, Kang CM, Chung TD
ACS applied materials & interfaces (2024) 163: 3082-3092. 138 302 ICC; tested species: rat

Generation of self-organized autonomic ganglion organoids from fibroblasts.
Liu S, Xiang K, Yuan F, Xiang M
iScience (2023) 263: 106241. 138 302 ICC; tested species: mouse

Conversion of Astrocytes and Fibroblasts into Functional Noradrenergic Neurons.
Li S, Shi Y, Yao X, Wang X, Shen L, Rao Z, Yuan J, Liu Y, Zhou Z, Zhang Z, Liu F, et al.
Cell reports (2019) 283: 682-697.e7. 138 302 ICC; tested species: mouse

Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons.
Martí Y, Matthaeus F, Lau T, Schloss P
Molecular and cellular neurosciences (2017) 83: 37-45. 138 302 ICC; tested species: mouse

Harnessing the trophic and modulatory potential of statins in a dopaminergic cell line.
Schmitt M, Dehay B, Bezard E, Garcia-Ladona FJ
Synapse (New York, N.Y.) (2016) 703: 71-86. 138 302 ICC

Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.
Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease (2023) 91: 35. 138 302 IHC; tested species: mouse

Characterization of Dmrt3-derived Neurons Suggest a Role within Locomotor Circuits.
Perry S, Larhammar M, Vieillard J, Nagaraja C, Hilscher MM, Tafreshiha A, Rofo F, Caixeta FV, Kullander K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 138 302 IHC; tested species: mouse

Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 138 302 IHC; tested species: mouse

Distribution of VGLUT3 in highly collateralized axons from the rat dorsal raphe nucleus as revealed by single-neuron reconstructions.
Gagnon D, Parent M
PloS one (2014) 92: e87709. 138 302 IHC; tested species: mouse

Sympathetic fiber sprouting in inflamed joints and adjacent skin contributes to pain-related behavior in arthritis.
Longo G, Osikowicz M, Ribeiro-da-Silva A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3324: 10066-74. 138 302 IHC

Cytoprotective effects of growth factors: BDNF more potent than GDNF in an organotypic culture model of Parkinson's disease.
Stahl K, Mylonakou MN, Skare Ø, Amiry-Moghaddam M, Torp R
Brain research (2011) 1378: 105-18. 138 302 IHC

GABA is localized in dopaminergic synaptic vesicles in the rodent striatum.
Stensrud MJ, Puchades M, Gundersen V
Brain structure & function (2014) 2196: 1901-12. 138 302 EM; tested species: rat

Cat. No.: 138 302

Amount: 200 µl

Price: $355.00

Generation of self-organized autonomic ganglion organoids from fibroblasts.
Liu S, Xiang K, Yuan F, Xiang M
iScience (2023) 263: 106241. 138 302 ICC; tested species: mouse

Harnessing the trophic and modulatory potential of statins in a dopaminergic cell line.
Schmitt M, Dehay B, Bezard E, Garcia-Ladona FJ
Synapse (New York, N.Y.) (2016) 703: 71-86. 138 302 ICC

GABA is localized in dopaminergic synaptic vesicles in the rodent striatum.
Stensrud MJ, Puchades M, Gundersen V
Brain structure & function (2014) 2196: 1901-12. 138 302 EM; tested species: rat

ICC

IHC

Background

Vesicular monoamine transporters VMaTs mediate the translocation of monoamines (serotonin, histamine, dopamine) from the cytoplasm into secretory vesicles by using a proton electrochemical gradient.
VMaTs are integral membrane proteins with 12 putative trans-membrane domains predicted by sequence analysis. Both, the N- and C-terminus of the proteins are located on the cytoplasmic side.
Two VMaT isoforms, VMaT 1 and VMaT 2, have been described. It has been proposed that VMaT 1 transports monoamines into large dense core vesicles (LDCVs), whereas VMaT 2 is needed for the loading of small synaptic vesicles (SSVs).
In rat VMaT 1 is expressed in the adrenal gland, while VMaT 2 is expressed in brain.

1 ) Jakobsen AM et al., J. Pathol. (2001)
2 ) Colliver TL et al., J. Neurosci. (2000)
3 ) Nirenberg MJ et al., Synapse (1997)
4 ) Nirenberg MJ et al., Proc. Natl. Acad. Sci. U.S.A. (1995)
5 ) Liu Y et al., J. Cell Biol. (1994)
6 ) Höltje M et al., J. Neurosci. (2000)

Protocols

ICC

IHC

Other products for this target

VMaT2 antibody C-terminus - 138 302

References for VMaT2 - 138 302