|Cat. No. 135 511||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks)
ICC: 1 : 100 up to 1 : 500 gallery
IHC: 1 : 100 gallery
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)|
Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
|Specificity||Specific for VGLUT 1. K.O.|
|Matching control protein/peptide||135-3P|
WB: This antibody yields weaker signals in Western blot experiments than 135 311, 135 011 and the polyclonal antibodies 135 302, 135 303, 135 304 and 135 306. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.