Tailor-made Antibodies
and Tools for Life Science

VGLUT1 antibody - 135 511 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 135 511
Amount: 100 µg
Price: $425.00
Cat. No. 135 511 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 gallery  
IHC-P: 1 : 100 up to 1 : 500 gallery  
Clone 317G6
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Epitop AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-3P
Remarks

WB: This antibody yields weaker signals in Western blot experiments than 135 311, 135 011 and the polyclonal antibodies 135 302, 135 303, 135 304 and 135 306. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_511.pdf

References for VGLUT1 - 135 511

A Neuron, Microglia, and Astrocyte Triple Co-culture Model to Study Alzheimer's Disease.
Luchena C, Zuazo-Ibarra J, Valero J, Matute C, Alberdi E, Capetillo-Zarate E
Frontiers in aging neuroscience (2022) 14: 844534. 135 511 WB; tested species: rat
Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology.
Argent L, Winter F, Prickett I, Carrasquero-Ordaz M, Olsen AL, Kramer H, Lancaster E, Becker EBE
The Journal of biological chemistry (2020) 29536: 12716-12726. 135 511 WB; tested species: mouse
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 135 511 WB; tested species: mouse
APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 135 511 WB
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 511 IHC, WB
Cat. No.: 135 511
Amount: 100 µg
Price: $425.00