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VGLUT 1 - 135 511

Glutamate transporter in the membrane of synaptic vesicles
Monoclonal mouse purified IgG
Cat. No.: 135 511
Amount: 100 µg
Price: $415.00
Cat. No. 135 511 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 100 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 500 gallery  
Clone 317G6
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Epitop Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1. K.O.
Matching control protein/peptide 135-3P
Remarks

WB: This antibody yields weaker signals in Western blot experiments than 135 311, 135 011 and the polyclonal antibodies 135 302, 135 303, 135 304 and 135 306. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_511.pdf

References for VGLUT 1 - 135 511

Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology.
Argent L, Winter F, Prickett I, Carrasquero-Ordaz M, Olsen AL, Kramer H, Lancaster E, Becker EBE
The Journal of biological chemistry (2020) 29536: 12716-12726. 135 511 WB; tested species: mouse
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 135 511 WB; tested species: mouse
APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 135 511 WB
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 511 WB, IHC
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 135 511 IP
Immunoprecipitation of Ribosome-Bound mRNAs from Astrocytic Perisynaptic Processes of the Mouse Hippocampus.
Mazaré N, Oudart M, Cheung G, Boulay AC, Cohen-Salmon M
STAR protocols (2020) 13: 100198. 135 511 ICC; tested species: mouse
Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity.
Kriebel M, Ebel J, Battke F, Griesbach S, Volkmer H
Frontiers in molecular neuroscience (2020) 13: 43. 135 511 ICC; tested species: rat
Presynaptic Endosomal Cathepsin D Regulates the Biogenesis of GABAergic Synaptic Vesicles.
Li X, Qin L, Li Y, Yu H, Zhang Z, Tao C, Liu Y, Xue Y, Zhang X, Xu Z, Wang Y, et al.
Cell reports (2019) 284: 1015-1028.e5. 135 511 ICC; tested species: rat
Presynaptic α2δ-2 Calcium Channel Subunits Regulate Postsynaptic GABAA Receptor Abundance and Axonal Wiring.
Geisler S, Schöpf CL, Stanika R, Kalb M, Campiglio M, Repetto D, Traxler L, Missler M, Obermair GJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3914: 2581-2605. 135 511 ICC; tested species: mouse
α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 135 511 ICC; tested species: mouse
CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.
Ricciardi S, Ungaro F, Hambrock M, Rademacher N, Stefanelli G, Brambilla D, Sessa A, Magagnotti C, Bachi A, Giarda E, Verpelli C, et al.
Nature cell biology (2012) 149: 911-23. 135 511 ICC; tested species: mouse
Postsynaptic TrkC and presynaptic PTPσ function as a bidirectional excitatory synaptic organizing complex.
Takahashi H, Arstikaitis P, Prasad T, Bartlett TE, Wang YT, Murphy TH, Craig AM
Neuron (2011) 692: 287-303. 135 511 ICC; tested species: rat
Telencephalic neurons monosynaptically link brainstem and forebrain premotor networks necessary for song.
Roberts TF, Klein ME, Kubke MF, Wild JM, Mooney R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2813: 3479-89. 135 511 ICC
Inhibition of Matrix Metalloproteinase 9 Activity Promotes Synaptogenesis in the Hippocampus.
Salamian A, Legutko D, Nowicka K, Badyra B, Kaźmierska-Grębowska P, Caban B, Kowalczyk T, Kaczmarek L, Beroun A
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 135 511 IHC; tested species: rat
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning.
Mazaré N, Oudart M, Moulard J, Cheung G, Tortuyaux R, Mailly P, Mazaud D, Bemelmans AP, Boulay AC, Blugeon C, Jourdren L, et al.
Cell reports (2020) 328: 108076. 135 511 IHC; tested species: mouse
Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor α-motoneurons: Anatomical and neurochemical study.
Grycz K, Głowacka A, Ji B, Czarkowska-Bauch J, Gajewska-Woźniak O, Skup M
PloS one (2019) 149: e0222849. 135 511 IHC; tested species: rat
Dorsal CA1 interneurons contribute to acute stress-induced spatial memory deficits.
Yu JY, Fang P, Wang C, Wang XX, Li K, Gong Q, Luo BY, Wang XD
Neuropharmacology (2018) : . 135 511 IHC; tested species: mouse
LRRTM1 underlies synaptic convergence in visual thalamus.
Monavarfeshani A, Stanton G, Van Name J, Su K, Mills WA, Swilling K, Kerr A, Huebschman NA, Su J, Fox MA
eLife (2018) 7: . 135 511 IHC; tested species: mouse
Distribution and development of molecularly distinct perineuronal nets in visual thalamus.
Sabbagh U, Monavarfeshani A, Su K, Zabet-Moghadam M, Cole J, Carnival E, Su J, Mirzaei M, Gupta V, Salekdeh GH, Fox MA, et al.
Journal of neurochemistry (2018) : . 135 511 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 135 511 IHC; tested species: mouse
P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 135 511 IHC; tested species: mouse
Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis.
Crèvecoeur J, Kaminski RM, Rogister B, Foerch P, Vandenplas C, Neveux M, Mazzuferi M, Kroonen J, Poulet C, Martin D, Sadzot B, et al.
Neuropathology and applied neurobiology (2014) 402: 191-204. 135 511 IHC; tested species: human
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 511 WB, IHC
Hippocampal hyperexcitability underlies enhanced fear memories in TgNTRK3, a panic disorder mouse model.
Santos M, D'Amico D, Spadoni O, Amador-Arjona A, Stork O, Dierssen M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3338: 15259-71. 135 511 IHC; tested species: mouse
BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu MS, Deogracias R, Gundelfinger ED, Kojima M, Nestel S, Frotscher M, Barde YA, et al.
The Journal of cell biology (2012) 1966: 775-88. 135 511 IHC
Combined Treatment With Environmental Enrichment and (-)-Epigallocatechin-3-Gallate Ameliorates Learning Deficits and Hippocampal Alterations in a Mouse Model of Down Syndrome.
Catuara-Solarz S, Espinosa-Carrasco J, Erb I, Langohr K, Gonzalez JR, Notredame C, Dierssen M
eNeuro () 35: . 135 511 IHC; tested species: mouse
Cat. No.: 135 511
Quantity: 100 µg
Price: $415.00
Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology.
Argent L, Winter F, Prickett I, Carrasquero-Ordaz M, Olsen AL, Kramer H, Lancaster E, Becker EBE
The Journal of biological chemistry (2020) 29536: 12716-12726. 135 511 WB; tested species: mouse
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 135 511 WB; tested species: mouse
APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 135 511 WB
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 511 WB, IHC
Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses.
Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3022: 7634-45. 135 511 IP
Immunoprecipitation of Ribosome-Bound mRNAs from Astrocytic Perisynaptic Processes of the Mouse Hippocampus.
Mazaré N, Oudart M, Cheung G, Boulay AC, Cohen-Salmon M
STAR protocols (2020) 13: 100198. 135 511 ICC; tested species: mouse
Interference With Complex IV as a Model of Age-Related Decline in Synaptic Connectivity.
Kriebel M, Ebel J, Battke F, Griesbach S, Volkmer H
Frontiers in molecular neuroscience (2020) 13: 43. 135 511 ICC; tested species: rat
Presynaptic Endosomal Cathepsin D Regulates the Biogenesis of GABAergic Synaptic Vesicles.
Li X, Qin L, Li Y, Yu H, Zhang Z, Tao C, Liu Y, Xue Y, Zhang X, Xu Z, Wang Y, et al.
Cell reports (2019) 284: 1015-1028.e5. 135 511 ICC; tested species: rat
Presynaptic α2δ-2 Calcium Channel Subunits Regulate Postsynaptic GABAA Receptor Abundance and Axonal Wiring.
Geisler S, Schöpf CL, Stanika R, Kalb M, Campiglio M, Repetto D, Traxler L, Missler M, Obermair GJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3914: 2581-2605. 135 511 ICC; tested species: mouse
α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 135 511 ICC; tested species: mouse
CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons.
Ricciardi S, Ungaro F, Hambrock M, Rademacher N, Stefanelli G, Brambilla D, Sessa A, Magagnotti C, Bachi A, Giarda E, Verpelli C, et al.
Nature cell biology (2012) 149: 911-23. 135 511 ICC; tested species: mouse
Postsynaptic TrkC and presynaptic PTPσ function as a bidirectional excitatory synaptic organizing complex.
Takahashi H, Arstikaitis P, Prasad T, Bartlett TE, Wang YT, Murphy TH, Craig AM
Neuron (2011) 692: 287-303. 135 511 ICC; tested species: rat
Telencephalic neurons monosynaptically link brainstem and forebrain premotor networks necessary for song.
Roberts TF, Klein ME, Kubke MF, Wild JM, Mooney R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2813: 3479-89. 135 511 ICC
Inhibition of Matrix Metalloproteinase 9 Activity Promotes Synaptogenesis in the Hippocampus.
Salamian A, Legutko D, Nowicka K, Badyra B, Kaźmierska-Grębowska P, Caban B, Kowalczyk T, Kaczmarek L, Beroun A
Cerebral cortex (New York, N.Y. : 1991) (2021) : . 135 511 IHC; tested species: rat
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning.
Mazaré N, Oudart M, Moulard J, Cheung G, Tortuyaux R, Mailly P, Mazaud D, Bemelmans AP, Boulay AC, Blugeon C, Jourdren L, et al.
Cell reports (2020) 328: 108076. 135 511 IHC; tested species: mouse
Early pre- and postsynaptic decrease in glutamatergic and cholinergic signaling after spinalization is not modified when stimulating proprioceptive input to the ankle extensor α-motoneurons: Anatomical and neurochemical study.
Grycz K, Głowacka A, Ji B, Czarkowska-Bauch J, Gajewska-Woźniak O, Skup M
PloS one (2019) 149: e0222849. 135 511 IHC; tested species: rat
Dorsal CA1 interneurons contribute to acute stress-induced spatial memory deficits.
Yu JY, Fang P, Wang C, Wang XX, Li K, Gong Q, Luo BY, Wang XD
Neuropharmacology (2018) : . 135 511 IHC; tested species: mouse
LRRTM1 underlies synaptic convergence in visual thalamus.
Monavarfeshani A, Stanton G, Van Name J, Su K, Mills WA, Swilling K, Kerr A, Huebschman NA, Su J, Fox MA
eLife (2018) 7: . 135 511 IHC; tested species: mouse
Distribution and development of molecularly distinct perineuronal nets in visual thalamus.
Sabbagh U, Monavarfeshani A, Su K, Zabet-Moghadam M, Cole J, Carnival E, Su J, Mirzaei M, Gupta V, Salekdeh GH, Fox MA, et al.
Journal of neurochemistry (2018) : . 135 511 IHC; tested species: mouse
Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.
Kaczmarek-Hajek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, Bruzzone S, Engel T, Jooss T, Krautloher A, Schuster S, et al.
eLife (2018) 7: . 135 511 IHC; tested species: mouse
P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb.
Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez-Gil DJ, Greer CA, Zufall F
Frontiers in cellular neuroscience (2018) 12: 295. 135 511 IHC; tested species: mouse
Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis.
Crèvecoeur J, Kaminski RM, Rogister B, Foerch P, Vandenplas C, Neveux M, Mazzuferi M, Kroonen J, Poulet C, Martin D, Sadzot B, et al.
Neuropathology and applied neurobiology (2014) 402: 191-204. 135 511 IHC; tested species: human
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 511 WB, IHC
Hippocampal hyperexcitability underlies enhanced fear memories in TgNTRK3, a panic disorder mouse model.
Santos M, D'Amico D, Spadoni O, Amador-Arjona A, Stork O, Dierssen M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3338: 15259-71. 135 511 IHC; tested species: mouse
BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
Dieni S, Matsumoto T, Dekkers M, Rauskolb S, Ionescu MS, Deogracias R, Gundelfinger ED, Kojima M, Nestel S, Frotscher M, Barde YA, et al.
The Journal of cell biology (2012) 1966: 775-88. 135 511 IHC
Combined Treatment With Environmental Enrichment and (-)-Epigallocatechin-3-Gallate Ameliorates Learning Deficits and Hippocampal Alterations in a Mouse Model of Down Syndrome.
Catuara-Solarz S, Espinosa-Carrasco J, Erb I, Langohr K, Gonzalez JR, Notredame C, Dierssen M
eNeuro () 35: . 135 511 IHC; tested species: mouse
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.