Tailor-made Antibodies
and Tools for Life Science

VGLUT1/2 antibody - 135 503

VGluT1 and 2 are glutamate transporters in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 135 503
Amount: 50 µg
Price: $385.00
Cat. No. 135 503 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100
IHC: yes gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 324 to 339 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: human (Q9P2U7, Q9P2U8), rat (Q62634, Q9JI12), mouse (Q3TXX4, Q8BLE7), mammals, chicken, zebrafish, frog.
Other species not tested yet.
Specificity Epitope identical in VGLUT 1 and VGLUT 2, one mismatch in VGLUT 3.
Remarks

This antibody is less powerful compared to those directed against the C-termini of VGLUTs but can be used for non-mammals like chicken and zebrafish.VGLUTs aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
WB: This antibody produces only a very weak and hardly detectable signal in westernblots on zebrafish brain derived samples.

Data sheet 135_503.pdf

References for VGLUT1/2 - 135 503

Visual Cortex Engagement in Retinitis Pigmentosa.
Pietra G, Bonifacino T, Talamonti D, Bonanno G, Sale A, Galli L, Baroncelli L
International journal of molecular sciences (2021) 2217: . 135 503 WB; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 135 503 WB, ICC; tested species: rat
Cat. No.: 135 503
Amount: 50 µg
Price: $385.00