Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

VGLUT 1/2 antibody - 135 503

VGluT1 and 2 are glutamate transporters in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 135 503
Amount: 50 µg
Price: $370.00
Cat. No. 135 503 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100
IHC: yes gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 324 to 339 from rat VGLUT1 (UniProt Id: Q62634)
Epitop Epitop: AA 324 to 339 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: human (Q9P2U7, Q9P2U8), rat (Q62634, Q9JI12), mouse (Q3TXX4, Q8BLE7), mammals, chicken, zebrafish, frog.
Other species not tested yet.
Specificity Epitope identical in VGLUT 1 and VGLUT 2, one mismatch in VGLUT 3.
Remarks

This antibody is less powerful compared to those directed against the C-termini of VGLUTs but can be used for non-mammals like chicken and zebrafish.VGLUTs aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
WB: This antibody produces only a very weak and hardly detectable signal in westernblots on zebrafish brain derived samples.

Data sheet 135_503.pdf

References for VGLUT 1/2 - 135 503

Visual Cortex Engagement in Retinitis Pigmentosa.
Pietra G, Bonifacino T, Talamonti D, Bonanno G, Sale A, Galli L, Baroncelli L
International journal of molecular sciences (2021) 2217: . 135 503 WB; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 135 503 WB, ICC; tested species: rat
16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis.
Tomasello DL, Kim JL, Khodour Y, McCammon JM, Mitalipova M, Jaenisch R, Futerman AH, Sive H
iScience (2022) 251: 103551. 135 503 ICC; tested species: human
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 135 503 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 135 503 WB, ICC; tested species: rat
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 135 503 ICC
CX3CR1 mutation alters synaptic and astrocytic protein expression, topographic gradients, and response latencies in the auditory brainstem.
Milinkeviciute G, Chokr SM, Castro EM, Cramer KS
The Journal of comparative neurology (2021) 52911: 3076-3097. 135 503 IHC; tested species: mouse
Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem.
Milinkeviciute G, Henningfield CM, Muniak MA, Chokr SM, Green KN, Cramer KS
Frontiers in neural circuits (2019) 13: 55. 135 503 IHC; tested species: mouse
Neurotransmitter Switching Regulated by miRNAs Controls Changes in Social Preference.
Dulcis D, Lippi G, Stark CJ, Do LH, Berg DK, Spitzer NC
Neuron (2017) 956: 1319-1333.e5. 135 503 IHC; tested species: frog
Cat. No.: 135 503
Amount: 50 µg
Price: $370.00
Visual Cortex Engagement in Retinitis Pigmentosa.
Pietra G, Bonifacino T, Talamonti D, Bonanno G, Sale A, Galli L, Baroncelli L
International journal of molecular sciences (2021) 2217: . 135 503 WB; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 135 503 WB, ICC; tested species: rat
16pdel lipid changes in iPSC-derived neurons and function of FAM57B in lipid metabolism and synaptogenesis.
Tomasello DL, Kim JL, Khodour Y, McCammon JM, Mitalipova M, Jaenisch R, Futerman AH, Sive H
iScience (2022) 251: 103551. 135 503 ICC; tested species: human
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 135 503 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 135 503 WB, ICC; tested species: rat
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 135 503 ICC
CX3CR1 mutation alters synaptic and astrocytic protein expression, topographic gradients, and response latencies in the auditory brainstem.
Milinkeviciute G, Chokr SM, Castro EM, Cramer KS
The Journal of comparative neurology (2021) 52911: 3076-3097. 135 503 IHC; tested species: mouse
Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem.
Milinkeviciute G, Henningfield CM, Muniak MA, Chokr SM, Green KN, Cramer KS
Frontiers in neural circuits (2019) 13: 55. 135 503 IHC; tested species: mouse
Neurotransmitter Switching Regulated by miRNAs Controls Changes in Social Preference.
Dulcis D, Lippi G, Stark CJ, Do LH, Berg DK, Spitzer NC
Neuron (2017) 956: 1319-1333.e5. 135 503 IHC; tested species: frog
Background

The vesicular glutamate transporter 1 , VGLUT 1 also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.