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Home > Products > 135 416

VGLUT2 antibody - 135 416

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Chicken polyclonal purified antibody
Cat. No.: 135 416
Amount: 50 µg
Price: $390.00
Cat. No. 135 416 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 5000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Matching control protein/peptide 135-4P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 135_416.pdf

References for VGLUT2 - 135 416

Behavioral decline in Shank3Δex4-22 mice during early adulthood parallels cerebellar granule cell glutamatergic synaptic changes.
Kshetri R, Beavers JO, Hyde R, Ewa R, Schwertman A, Porcayo S, Richardson BD
Molecular autism (2024) 151: 52. 135 416 IHC; tested species: mouse
Synaptic circuits involving gastrin-releasing peptide receptor-expressing neurons in the dorsal horn of the mouse spinal cord.
Quillet R, Gutierrez-Mecinas M, Polgár E, Dickie AC, Boyle KA, Watanabe M, Todd AJ
Frontiers in molecular neuroscience (2023) 16: 1294994. 135 416 IHC; tested species: mouse
Glutamate delta 1 receptor regulates autophagy mechanisms and affects excitatory synapse maturation in the somatosensory cortex.
Gawande DY, S Narasimhan KK, Bhatt JM, Pavuluri R, Kesherwani V, Suryavanshi PS, Shelkar GP, Dravid SM
Pharmacological research (2022) 178: 106144. 135 416 IHC; tested species: mouse
Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 135 416 IHC; tested species: mouse
Local targets of T-stellate cells in the ventral cochlear nucleus.
Lin L, Campbell J, Oertel D, Smith PH
The Journal of comparative neurology (2022) : . 135 416 IHC; tested species: mouse
Antibodies Against the Gastrin-releasing Peptide Precursor Pro-Gastrin-releasing Peptide Reveal Its Expression in the Mouse Spinal Dorsal Horn.
Gutierrez-Mecinas M, Kókai É, Polgár E, Quillet R, Titterton HF, Weir GA, Watanabe M, Todd AJ
Neuroscience (2022) 510: 60-71. 135 416 IHC; tested species: mouse
EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization.
Wang Y, Eddison M, Fleishman G, Weigert M, Xu S, Wang T, Rokicki K, Goina C, Henry FE, Lemire AL, Schmidt U, et al.
Cell (2021) : . 135 416 IHC; tested species: mouse
Smad4-dependent morphogenic signals control the maturation and axonal targeting of basal vomeronasal sensory neurons to the accessory olfactory bulb.
Naik AS, Lin JM, Taroc EZM, Katreddi RR, Frias JA, Lemus AA, Sammons MA, Forni PE
Development (Cambridge, England) (2020) 1478: . 135 416 IHC; tested species: mouse
Optogenetic "low-theta" pacing of the septohippocampal circuit is sufficient for spatial goal finding and is influenced by behavioral state and cognitive demand.
Mouchati PR, Kloc ML, Holmes GL, White SL, Barry JM
Hippocampus (2020) 3011: 1167-1193. 135 416 IHC; tested species: rat
Circuit dissection of the role of somatostatin in itch and pain.
Huang J, Polgár E, Solinski HJ, Mishra SK, Tseng PY, Iwagaki N, Boyle KA, Dickie AC, Kriegbaum MC, Wildner H, Zeilhofer HU, et al.
Nature neuroscience (2018) : . 135 416 IHC; tested species: mouse
Cat. No.: 135 416
Amount: 50 µg
Price: $390.00
WB
ICC
IHC
IHC-P
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