Tailor-made Antibodies
and Tools for Life Science

VGLUT1 antibody - 135 316 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Chicken polyclonal IgY fraction
Cat. No.: 135 316
Amount: 200 µl
Price: $370.00
Cat. No. 135 316 200 µl antibody, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
IHC-Fr: 1 : 500 up to 1 : 2000 (see remarks) gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-0P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-Fr: PFA fixation is recommended.

Data sheet 135_316.pdf

References for VGLUT1 - 135 316

Ciliary neuropeptidergic signaling dynamically regulates excitatory synapses in postnatal neocortical pyramidal neurons.
Tereshko L, Gao Y, Cary BA, Turrigiano GG, Sengupta P
eLife (2021) 10: . 135 316 ICC; tested species: rat
Isolation of Human CD49f+ Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays.
Barbar L, Rusielewicz T, Zimmer M, Kalpana K, Fossati V
STAR protocols (2020) 13: 100172. 135 316 ICC; tested species: human
Cat. No.: 135 316
Amount: 200 µl
Price: $370.00