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VGLUT 1 - 135 316

Glutamate transporter in the membrane of synaptic vesicles
Polyclonal chicken IgY fraction
Cat. No.: 135 316
Amount: 200 µl
Price: $350.00
Cat. No. 135 316 200 µl antibody, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1. K.O.
Matching control protein/peptide 135-0P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_316.pdf

References for VGLUT 1 - 135 316

Isolation of Human CD49f+ Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays.
Barbar L, Rusielewicz T, Zimmer M, Kalpana K, Fossati V
STAR protocols (2020) 13: 100172. 135 316 ICC; tested species: human
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 135 316 IHC; tested species: mouse
AAV9-Mediated Delivery of miR-23a Reduces Disease Severity in Smn2B-/SMA Model Mice.
Kaifer KA, Villalón E, O'Brien BS, Sison SL, Smith CE, Simon ME, Marquez J, O'Day S, Hopkins AE, Neff R, Rindt H, et al.
Human molecular genetics (2019) : . 135 316 IHC; tested species: mouse
Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease.
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, Dick HB, Faissner A, Joachim SC
International journal of molecular sciences (2019) 2010: . 135 316 IHC; tested species: mouse
Presynaptic PRRT2 deficiency causes cerebellar dysfunction and paroxysmal kinesigenic dyskinesia.
Calame DJ, Xiao J, Khan MM, Hollinsworth TJ, Xue Y, Person AL, LeDoux MS
Neuroscience (2020) : . 135 316 IHC-P; tested species: mouse
Cat. No.: 135 316
Quantity: 200 µl
Price: $350.00
Isolation of Human CD49f+ Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays.
Barbar L, Rusielewicz T, Zimmer M, Kalpana K, Fossati V
STAR protocols (2020) 13: 100172. 135 316 ICC; tested species: human
Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 135 316 IHC; tested species: mouse
AAV9-Mediated Delivery of miR-23a Reduces Disease Severity in Smn2B-/SMA Model Mice.
Kaifer KA, Villalón E, O'Brien BS, Sison SL, Smith CE, Simon ME, Marquez J, O'Day S, Hopkins AE, Neff R, Rindt H, et al.
Human molecular genetics (2019) : . 135 316 IHC; tested species: mouse
Transfer of the Experimental Autoimmune Glaucoma Model from Rats to Mice-New Options to Study Glaucoma Disease.
Reinehr S, Reinhard J, Wiemann S, Hesse K, Voss C, Gandej M, Dick HB, Faissner A, Joachim SC
International journal of molecular sciences (2019) 2010: . 135 316 IHC; tested species: mouse
Presynaptic PRRT2 deficiency causes cerebellar dysfunction and paroxysmal kinesigenic dyskinesia.
Calame DJ, Xiao J, Khan MM, Hollinsworth TJ, Xue Y, Person AL, LeDoux MS
Neuroscience (2020) : . 135 316 IHC-P; tested species: mouse
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.