|Cat. No. 135 316||200 µl antibody, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 500 gallery
|Immunogen||Synthetic peptide corresponding to AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)|
Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
|Specificity||Specific for VGLUT 1. K.O.|
|Matching control protein/peptide||135-0P|
This antibody is highly recommended as a marker for glutamatergic nerve terminals.
The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.