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VGLUT1 antibody - 135 309 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Chicken monoclonal recombinant IgY
Cat. No.: 135 309
Amount: 50 µg
Price: $415.00
Cat. No. 135 309 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10 000 (AP-staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
Clone Ch68B7
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT 1. (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity K.O. validated
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 68B7. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_309.pdf
Cat. No.: 135 309
Amount: 50 µg
Price: $415.00
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.