|Cat. No. 135 307||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 1000 up to 1 : 10000 gallery
IHC: 1 : 300 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)|
Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
|Specificity||Specific for VGLUT 1. K.O.|
|Matching control protein/peptide||135-3P|
This antibody is highly recommended as a marker for glutamatergic nerve terminals.
The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.