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VGLUT 1 - 135 307

Glutamate transporter in the membrane of synaptic vesicles
Polyclonal goat antiserum
Cat. No.: 135 307
Amount: 200 µl
Price: $330.00
Cat. No. 135 307 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 up to 1 : 10000 gallery  
IHC: 1 : 300 gallery  
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1. K.O.
Matching control protein/peptide 135-3P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
This antibody is highly recommended as marker for glutamatergic nerve terminals and gives excellent results in ICC.

Data sheet 135_307.pdf

References for VGLUT 1 - 135 307

Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation.
Stavsky A, Stoler O, Kostic M, Katoshevsky T, Assali EA, Savic I, Amitai Y, Prokisch H, Leiz S, Daumer-Haas C, Fleidervish I, et al.
Communications biology (2021) 41: 666. 135 307 ICC; tested species: mouse
PSD-95 promotes the stabilization of young synaptic contacts.
Taft CE, Turrigiano GG
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691633: 20130134. 135 307 ICC; tested species: rat
Puncta of neuronal nitric oxide synthase (nNOS) mediate NMDA-receptor signalling in the auditory midbrain.
Olthof BM, Gartside SE, Rees A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 135 307 IHC
Cat. No.: 135 307
Quantity: 200 µl
Price: $330.00
Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation.
Stavsky A, Stoler O, Kostic M, Katoshevsky T, Assali EA, Savic I, Amitai Y, Prokisch H, Leiz S, Daumer-Haas C, Fleidervish I, et al.
Communications biology (2021) 41: 666. 135 307 ICC; tested species: mouse
PSD-95 promotes the stabilization of young synaptic contacts.
Taft CE, Turrigiano GG
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691633: 20130134. 135 307 ICC; tested species: rat
Puncta of neuronal nitric oxide synthase (nNOS) mediate NMDA-receptor signalling in the auditory midbrain.
Olthof BM, Gartside SE, Rees A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 135 307 IHC
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.