|Cat. No. 135 303C3||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Sulfo-Cyanine 3. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 500 up to 1 : 10000 gallery
IHC: 1 : 500 up to 1 : 1000 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 456 to 560 from rat VGLUT1 (UniProt Id: Q62634)|
Reacts with: human (Q9P2U7), rat (Q62634), mouse (Q3TXX4), cow, goat, sheep, dog.
Other species not tested yet.
|Specificity||Specific for VGLUT 1. K.O.|
|Matching control protein/peptide||135-3P|
This antibody is highly recommended as markers for glutamatergic nerve terminals.
The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.