Synaptic Systems

Cat. No.: 135 302

Amount: 200 µl

Price: $365.00

Cat. No. 135 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 1000 up to 1 : 10000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 gallery ExM: yes (see remarks) gallery
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity	Reacts with: human (Q9P2U7), rat (Q62634), mouse (Q3TXX4), cow, goat, dog, sheep, ape. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	135-3P
Remarks	This antibody is highly recommended as a marker for glutamatergic nerve terminals. WB: VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ExM: This antibody has been successfully used for the epitope-preserving magnified analysis of the proteome (eMAP) expansion microscopy method (Park et al. 2021. PMID: 34767453). Variance from the standard protocol: Expansion solution for imaging: 0.02X PBS
Data sheet	135_302.pdf

References for VGLUT1 - 135 302

Astragaloside IV ameliorates autism-like behaviors in BTBR mice by modulating Camk2n2-dependent OXPHOS and neurotransmission in the mPFC.
Chen M, Shi J, Liu T, Liu J, Liu Y, Li J, Luo Y, Luo J, Li X, Gong H, Fan X, et al.
Journal of advanced research (2025) : . 135 302 WB; tested species: mouse

The TrkC-PTPσ complex governs synapse maturation and anxiogenic avoidance via synaptic protein phosphorylation.
Khaled H, Ghasemi Z, Inagaki M, Patel K, Naito Y, Feller B, Yi N, Bourojeni FB, Lee AK, Chofflet N, Kania A, et al.
The EMBO journal (2024) : . 135 302 WB; tested species: mouse

Microglia mitochondrial complex I deficiency during development induces glial dysfunction and early lethality.
Mora-Romero B, Capelo-Carrasco N, Pérez-Moreno JJ, Alvarez-Vergara MI, Trujillo-Estrada L, Romero-Molina C, Martinez-Marquez E, Morano-Catalan N, Vizuete M, Lopez-Barneo J, Nieto-Gonzalez JL, et al.
Nature metabolism (2024) : . 135 302 WB; tested species: mouse

Adult-onset deactivation of autophagy leads to loss of synapse homeostasis and cognitive impairment, with implications for alzheimer disease.
Grosso Jasutkar H, Wasserlein EM, Ishola A, Litt N, Staniszewski A, Arancio O, Yamamoto A
Autophagy (2024) : 1-16. 135 302 WB; tested species: mouse

Postweaning Development Influences Endogenous VPAC1 Modulation of LTP Induced by Theta-Burst Stimulation: A Link to Maturation of the Hippocampal GABAergic System.
Gil M, Caulino-Rocha A, Bento M, Rodrigues NC, Silva-Cruz A, Ribeiro JA, Cunha-Reis D
Biomolecules (2024) 143: . 135 302 WB; tested species: rat

Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 135 302 WB; tested species: mouse

Presynapses contain distinct actin nanostructures.
Bingham D, Jakobs CE, Wernert F, Boroni-Rueda F, Jullien N, Schentarra EM, Friedl K, Da Costa Moura J, van Bommel DM, Caillol G, Ogawa Y, et al.
The Journal of cell biology (2023) 22210: . 135 302 WB; tested species: rat

Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 135 302 WB, IHC; tested species: mouse

Cerebellar developmental deficits underlie neurodegenerative disorder spinocerebellar ataxia type 23.
Smeets CJLM, Ma KY, Fisher SE, Verbeek DS
Brain pathology (Zurich, Switzerland) (2021) 312: 239-252. 135 302 WB; tested species: mouse

Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose.
Qian C, Wu Z, Sun R, Yu H, Zeng J, Rao Y, Li Y
eLife (2021) 10: . 135 302 WB; tested species: mouse

Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.
Cheret C, Ganzella M, Preobraschenski J, Jahn R, Ahnert-Hilger G
Cell reports (2021) 342: 108623. 135 302 WB, ICC; tested species: human,mouse

Protein changes in synaptosomes of Huntington's disease knock-in mice are dependent on age and brain region.
Sapp E, Seeley C, Iuliano M, Weisman E, Vodicka P, DiFiglia M, Kegel-Gleason KB
Neurobiology of disease (2020) 141: 104950. 135 302 WB; tested species: mouse

Mechanoceutics Alters Alzheimer's Disease Phenotypes in Transgenic Rats: A Pilot Study.
Tobey H, Lucas T, Paul S, Berr SS, Mehrkens B, Brolinson PG, Klein BG, Costa BM
Journal of Alzheimer's disease : JAD (2020) 742: 421-427. 135 302 WB; tested species: rat

GABA, but Not Bestrophin-1, Is Localized in Astroglial Processes in the Mouse Hippocampus and the Cerebellum.
Ormel L, Lauritzen KH, Schreiber R, Kunzelmann K, Gundersen V
Frontiers in molecular neuroscience (2020) 13: 135. 135 302 WB; tested species: mouse

Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates.
Wu X, Ganzella M, Zhou J, Zhu S, Jahn R, Zhang M
Molecular cell (2020) : . 135 302 WB

A complement-microglial axis driving inhibitory synapse related protein loss might contribute to systemic inflammation-induced cognitive impairment.
Li SM, Li B, Zhang L, Zhang GF, Sun J, Ji MH, Yang JJ
International immunopharmacology (2020) 87: 106814. 135 302 WB, IHC; tested species: mouse

Merlin modulates process outgrowth and synaptogenesis in the cerebellum.
Toledo A, Lang F, Doengi M, Morrison H, Stein V, Baader SL
Brain structure & function (2019) : . 135 302 WB; tested species: mouse

SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior.
Soiza-Reilly M, Meye FJ, Olusakin J, Telley L, Petit E, Chen X, Mameli M, Jabaudon D, Sze JY, Gaspar P
Molecular psychiatry (2018) : . 135 302 WB; tested species: mouse

Piperlongumine decreases cognitive impairment and improves hippocampal function in aged mice.
Go J, Park TS, Han GH, Park HY, Ryu YK, Kim YH, Hwang JH, Choi DH, Noh JR, Hwang DY, Kim S, et al.
International journal of molecular medicine (2018) 424: 1875-1884. 135 302 WB; tested species: mouse

VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context.
Richter K, Schmutz I, Darna M, Zander JF, Chavan R, Albrecht U, Ahnert-Hilger G
Neuroscience (2018) 371: 29-37. 135 302 WB; tested species: mouse

IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
Tanabe Y, Naito Y, Vasuta C, Lee AK, Soumounou Y, Linhoff MW, Takahashi H
Nature communications (2017) 81: 408. 135 302 WB

Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits.
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, Su YA, Li K, Si T
Frontiers in behavioral neuroscience (2017) 11: 260. 135 302 WB; tested species: rat

Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 135 302 WB; tested species: mouse

Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, Rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 302 IP, WB; tested species: mouse

Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model.
Park TS, Ryu YK, Park HY, Kim JY, Go J, Noh JR, Kim YH, Hwang JH, Choi DH, Oh WK, Lee CH, et al.
International journal of molecular medicine (2017) 391: 21-30. 135 302 WB

Cerebellar synaptogenesis is compromised in mouse models of DYT1 dystonia.
Vanni V, Puglisi F, Bonsi P, Ponterio G, Maltese M, Pisani A, Mandolesi G
Experimental neurology (2015) 271: 457-67. 135 302 WB, ICC, IHC; tested species: mouse

REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation.
Garcia-Manteiga JM, Bonfiglio S, Folladori L, Malosio ML, Lazarevic D, Stupka E, Cittaro D, Meldolesi J
Frontiers in cellular neuroscience (2015) 9: 438. 135 302 WB

Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 135 302 IHC, WB

Regulation of glutamate release by heteromeric nicotinic receptors in layer V of the secondary motor region (Fr2) in the dorsomedial shoulder of prefrontal cortex in mouse.
Aracri P, Amadeo A, Pasini ME, Fascio U, Becchetti A
Synapse (New York, N.Y.) (2013) 676: 338-57. 135 302 WB, IHC; tested species: mouse

Simultaneous monitoring of presynaptic transmitter release and postsynaptic receptor trafficking reveals an enhancement of presynaptic activity in metabotropic glutamate receptor-mediated long-term depression.
Xu W, Tse YC, Dobie FA, Baudry M, Craig AM, Wong TP, Wang YT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3313: 5867-5877. 135 302 WB

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 135 302 WB; tested species: mouse

Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny.
Romero-Alemán MM, Monzón-Mayor M, Santos E, Lang DM, Yanes C
The Journal of comparative neurology (2012) 52010: 2163-84. 135 302 WB, IHC

Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 135 302 WB; tested species: mouse