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VGLUT 3 antibody - 135 211

VGluT3 is a synaptic vesicle glutamate transporter
Mouse monoclonal purified IgG
Cat. No.: 135 211
Amount: 100 µg
Price: $415.00
Cat. No. 135 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 up to 1 : 500 gallery  
Clone 57A8
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse VGLUT 3 (UniProt Id: Q8BFU8)
Reactivity Reacts with: rat (Q7TSF2), mouse (Q8BFU8).
Other species not tested yet.
Specificity Specific for VGLUT 3
Remarks

WB: Due to the low abundance of this protein in the brain, immunoblotting is difficult.

Data sheet 135_211.pdf

References for VGLUT 3 - 135 211

Early-life sevoflurane exposure impairs fear memory by suppressing extracellular signal-regulated kinase signaling in the bed nucleus of stria terminalis GABAergic neurons.
Zhao XP, Zhong F, Luo RY, Zhang YL, Luo C, Li H, Dai RP
Neuropharmacology (2021) 191: 108584. 135 211 IHC; tested species: mouse
Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 135 211 IHC, EM; tested species: rat
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 211 UPTAKE; tested species: rat
Cat. No.: 135 211
Amount: 100 µg
Price: $415.00
Early-life sevoflurane exposure impairs fear memory by suppressing extracellular signal-regulated kinase signaling in the bed nucleus of stria terminalis GABAergic neurons.
Zhao XP, Zhong F, Luo RY, Zhang YL, Luo C, Li H, Dai RP
Neuropharmacology (2021) 191: 108584. 135 211 IHC; tested species: mouse
Synaptic Organization of VGLUT3 Expressing Low-Threshold Mechanosensitive C Fiber Terminals in the Rodent Spinal Cord.
Larsson M, Broman J
eNeuro () 61: . 135 211 IHC, EM; tested species: rat
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 211 UPTAKE; tested species: rat
Background

The vesicular glutamate transporter 3 VGLUT 3 is closely related to VGLUT 1 and VGLUT 2 by sequence similarity. However, VGLUT 3 defines a new distinct glutamatergic system in brain which is strictly seperated from VGLUT 1 and VGLUT 2 synapses. Co-localization with the acetylcholine transporter VAChT and the monoamine transporter 2 VMaT 2 has been observed.