Syntenin antibody - 133 011

Syntenin is an ubiquitously expressed PDZ domain protein

Mouse monoclonal purified IgG

Cat. No.: 133 011

Amount: 100 µg

Price: $420.00

Cat. No. 133 011	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 (ECL detection) (see remarks) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	32A6
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 298 from human Syntenin1 (UniProt Id: O00560)
Reactivity	Reacts with: human (O00560, Q9H190). Weaker signal: rat (Q9JI92, Q4KLN0), mouse (O08992, Q99JZ0). Other species not tested yet.
Specificity	Recognizes syntenin 1 and 2.
Matching control protein/peptide	133-0P
Remarks	WB: Sensitive detection methods like ECL are required to detect endogenous syntenin by WB.
Data sheet	Datasheet 133_011

Cat. No.: 133 011

Amount: 100 µg

Price: $420.00

ICC

Background

Syntenin is an ubiquitously expressed PDZ domain protein that binds to the cytoplasmic tails of several cell surface proteins. It has been particularly implicated in cell-cell interactions in the brain via its interactions with proteins such as syndecans, ephrins and neurofascin.

1 ) Grootjans JJ et al., J. Biol. Chem. (2000)
2 ) Fernández-Larrea J et al., Mol. Cell (1999)
3 ) Fialka I et al., J. Biol. Chem. (1999)
4 ) Grootjans JJ et al., Proc. Natl. Acad. Sci. U.S.A. (1997)

Protocols

ICC

Fixed Cells

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