pH-sensitive probe fluorescent only under acidic conditions
|Cat. No. 131 103CpH||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E
CypHer5E is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E can be detected with the filter sets for Cy5TM.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 100 up to 1 : 200 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 510 to 525 from rat VGAT (UniProt Id: O35458)|
Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
|Specificity||Specific for VGAT. K.O.|
This antibody is intended for direct labeling of recycling synaptic vesicles at inhibitory nerve terminals in living primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. Further details see Martens et al. 2008.