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Home > Products > 131 103CpH

VGAT antibody luminal domain - 131 103CpH K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles

pH-sensitive probe fluorescent only under acidic conditions

Rabbit polyclonal purified antibody
Cat. No.: 131 103CpH
Amount: 50 µg
Price: $785.00
Cat. No. 131 103CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label CypHer5E
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: This antibody is intended for direct labeling of recycling synaptic vesicles at inhibitory nerve terminals in living primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. Further details see Martens et al. 2008.
Data sheet 131_103cph.pdf

References for VGAT - 131 103CpH

Rho GTPase signaling and mDia facilitate endocytosis via presynaptic actin.
Oevel K, Hohensee S, Kumar A, Rosas-Brugada I, Bartolini F, Soykan T, Haucke V
eLife (2024) 12: . 131 103CpH UPTAKE; tested species: mouse
Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses.
López-Hernández T, Takenaka KI, Mori Y, Kongpracha P, Nagamori S, Haucke V, Takamori S
eLife (2022) 11: . 131 103CpH UPTAKE; tested species: mouse
Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.
Park C, Chen X, Tian CL, Park GN, Chenouard N, Lee H, Yeo XY, Jung S, Tsien RW, Bi GQ, Park H, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1189: . 131 103CpH UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 131 103CpH UPTAKE; tested species: rat
A readily retrievable pool of synaptic vesicles.
Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J
Nature neuroscience (2011) 147: 833-9. 131 103CpH UPTAKE
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103CpH UPTAKE; tested species: rat
Cat. No.: 131 103CpH
Amount: 50 µg
Price: $785.00
ICC
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