Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 131 103CpH

VGAT antibody luminal domain - 131 103CpH K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles

pH-sensitive probe fluorescent only under acidic conditions

Rabbit polyclonal purified antibody
Cat. No.: 131 103CpH
Amount: 50 µg
Price: $780.00
Cat. No. 131 103CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.

CypHer5E® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E® can be detected with the filter sets for Cy5®.

CypHer5E®: λex 647 nm / λem 667 nm

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label CypHer5E
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: This antibody is intended for direct labeling of recycling synaptic vesicles at inhibitory nerve terminals in living primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. Further details see Martens et al. 2008.
Data sheet 131_103cph.pdf

References for VGAT - 131 103CpH

Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses.
López-Hernández T, Takenaka KI, Mori Y, Kongpracha P, Nagamori S, Haucke V, Takamori S
eLife (2022) 11: . 131 103CpH UPTAKE; tested species: mouse
Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.
Park C, Chen X, Tian CL, Park GN, Chenouard N, Lee H, Yeo XY, Jung S, Tsien RW, Bi GQ, Park H, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1189: . 131 103CpH UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 131 103CpH UPTAKE; tested species: rat
A readily retrievable pool of synaptic vesicles.
Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J
Nature neuroscience (2011) 147: 833-9. 131 103CpH UPTAKE
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103CpH UPTAKE; tested species: rat
A common origin of synaptic vesicles undergoing evoked and spontaneous fusion.
Hua Y, Sinha R, Martineau M, Kahms M, Klingauf J
Nature neuroscience (2010) 1312: 1451-3. 131 103CpH
Cat. No.: 131 103CpH
Amount: 50 µg
Price: $780.00
Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses.
López-Hernández T, Takenaka KI, Mori Y, Kongpracha P, Nagamori S, Haucke V, Takamori S
eLife (2022) 11: . 131 103CpH UPTAKE; tested species: mouse
Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.
Park C, Chen X, Tian CL, Park GN, Chenouard N, Lee H, Yeo XY, Jung S, Tsien RW, Bi GQ, Park H, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1189: . 131 103CpH UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 131 103CpH UPTAKE; tested species: rat
A readily retrievable pool of synaptic vesicles.
Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J
Nature neuroscience (2011) 147: 833-9. 131 103CpH UPTAKE
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103CpH UPTAKE; tested species: rat
A common origin of synaptic vesicles undergoing evoked and spontaneous fusion.
Hua Y, Sinha R, Martineau M, Kahms M, Klingauf J
Nature neuroscience (2010) 1312: 1451-3. 131 103CpH
ICC
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.
Other products for this target