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VGAT lumenal domain - 131 103C2

GABA transporter in the membrane of synaptic vesicles
Polyclonal rabbit purified antibody
Cat. No.: 131 103C2
Amount: 50 µg
Price: 775.00 €
Cat. No. 131 103C2 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Sulfo-Cyanine 2. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
Label Sulfo-Cyanine 2
Immunogen Synthetic peptide corresponding to AA 510 to 525 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT. K.O.
Remarks This antibody is intended for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding to living neurons. Further details see Martens et al. 2008.
Data sheet 131_103c2.pdf

References for VGAT - 131 103C2

Key physiological parameters dictate triggering of activity-dependent bulk endocytosis in hippocampal synapses.
Wenzel EM, Morton A, Ebert K, Welzel O, Kornhuber J, Cousin MA, Groemer TW
PloS one (2012) 76: e38188. 131 103C2 UPTAKE
Stabilization of GABA(A) receptors at endocytic zones is mediated by an AP2 binding motif within the GABA(A) receptor β3 subunit.
Smith KR, Muir J, Rao Y, Browarski M, Gruenig MC, Sheehan DF, Haucke V, Kittler JT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 327: 2485-98. 131 103C2 UPTAKE
Cat. No.: 131 103C2
Quantity: 50 µg
Price: 775.00 €
Key physiological parameters dictate triggering of activity-dependent bulk endocytosis in hippocampal synapses.
Wenzel EM, Morton A, Ebert K, Welzel O, Kornhuber J, Cousin MA, Groemer TW
PloS one (2012) 76: e38188. 131 103C2 UPTAKE
Stabilization of GABA(A) receptors at endocytic zones is mediated by an AP2 binding motif within the GABA(A) receptor β3 subunit.
Smith KR, Muir J, Rao Y, Browarski M, Gruenig MC, Sheehan DF, Haucke V, Kittler JT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 327: 2485-98. 131 103C2 UPTAKE
Background
The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as the vesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.