Tailor-made Antibodies
and Tools for Life Science

VGAT antibody luminal domain - 131 103C2 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 131 103C2
Amount: 50 µg
Price: $785.00
Cat. No. 131 103C2 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 2.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 200 (see remarks) gallery  
IHC: yes
IHC-P: not tested yet
Label Cyanine 2
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: This antibody is intended for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding to living neurons. Further details see Martens et al. 2008.

Data sheet 131_103c2.pdf

References for VGAT - 131 103C2

Clathrin-independent endocytic retrieval of SV proteins mediated by the clathrin adaptor AP-2 at mammalian central synapses.
López-Hernández T, Takenaka KI, Mori Y, Kongpracha P, Nagamori S, Haucke V, Takamori S
eLife (2022) 11: . 131 103C2 ICC; tested species: mouse
Cat. No.: 131 103C2
Amount: 50 µg
Price: $785.00