|Cat. No. 131 103||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 100 up to 1 : 200 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 510 to 525 from rat VGAT (UniProt Id: O35458)|
Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
|Specificity||Specific for VGAT. K.O. PubMed: 19052203|
VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.These antibodies are intended for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding to living neurons. Further details see Martens et al. 2008.