Tailor-made Antibodies
and Tools for Life Science

VGAT antibody luminal domain - 131 103 K.O.

VGAT or VIAAT is the GABA and glycine transporter in the membrane of synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 131 103
Amount: 50 µg
Price: $665.00
Cat. No. 131 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 200 (see remarks) gallery  
IHC: 1 : 100 up to 1 : 500
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity K.O. validated PubMed: 19052203
Remarks

WB: To avoid protein aggregation, do not heat samples for SDS-PAGE.
ICC: This antibody can also be used for labeling of recycling synaptic vesicles in living neurons.
Further details see Martens et al. 2008.

Data sheet 131_103.pdf

References for VGAT - 131 103

A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles.
Gopal N, Leitz J, Wang C, Esquivies L, Pfuetzner RA, Brunger AT
Current research in physiology (2024) 7: 100121. 131 103 WB; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 131 103 WB, ICC, IHC, UPTAKE; KO verified; tested species: mouse,rat
Cat. No.: 131 103
Amount: 50 µg
Price: $665.00