Cat. No.: 130 011
Amount: 100 µg
Price:
$415.00
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Cat. No. 130 011 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not recommended Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet |
Clone | 81.2 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 447 from bovine Rab-GDI1 (UniProt Id: P21856) |
Reactivity |
Reacts with: human (P31150), rat (P50398), mouse (P50396), hamster, cow. Other species not tested yet. |
Specificity | Recognizes Rab-GDI-α and -β. |
Remarks |
Immunoprecipitates Rab-GDI complexes. |
Data sheet | 130_011.pdf |
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Rab-GDI forms complexes exclusively with the GDP-form of rab proteins. Complex formation shields the geranylgeranyl moieties of rabs and leads to their dissociation from the membrane.
There are several isoforms of rab-GDI which nondiscriminately interact with all rab proteins including rab 3 and rab 5 but not with other small GTPases such as members of the rho or ras families. GDI-α is predominantly expressed in brain whereas other isoforms are more generally distributed.