Synaptic Systems - Neuroligin1

Cat. No.: 129 013

Amount: 50 µg

Price: $375.00

Cat. No. 129 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 737 to 754 from mouse Neuroligin1 (UniProt Id: Q99K10)
Reactivity	Reacts with: rat (Q62765), mouse (Q99K10). Other species not tested yet.
Specificity	K.O. validated
Data sheet	129_013.pdf

References for Neuroligin1 - 129 013

Cortical reorganization of the glutamate synapse in the activity-based anorexia rat model: Impact on cognition.
Mottarlini F, Targa G, Bottan G, Tarenzi B, Fumagalli F, Caffino L
Journal of neurochemistry (2022) 1614: 350-365. 129 013 WB; tested species: rat

Responsivity of serotonin transporter knockout rats to short and long access to cocaine: Modulation of the glutamate signalling in the nucleus accumbens shell.
Caffino L, Mottarlini F, Targa G, Verheij MMM, Fumagalli F, Homberg JR
British journal of pharmacology (2022) 17914: 3727-3739. 129 013 WB; tested species: rat

Hevin-calcyon interaction promotes synaptic reorganization after brain injury.
Kim JH, Jung HG, Kim A, Shim HS, Hyeon SJ, Lee YS, Han J, Jung JH, Lee J, Ryu H, Park JY, et al.
Cell death and differentiation (2021) 289: 2571-2588. 129 013 WB; tested species: mouse

A sex difference in the response of the rodent postsynaptic density to synGAP haploinsufficiency.
Mastro TL, Preza A, Basu S, Chattarji S, Till SM, Kind PC, Kennedy MB
eLife (2020) 9: . 129 013 WB; tested species: rat

Prefrontal Nectin3 Reduction Mediates Adolescent Stress-Induced Deficits of Social Memory, Spatial Working Memory, and Dendritic Structure in Mice.
Wang HL, Li JT, Wang H, Sun YX, Liu R, Wang XD, Su YA, Si TM
Neuroscience bulletin (2020) : . 129 013 WB; tested species: mouse

Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation.
Letellier M, Lagardère M, Tessier B, Janovjak H, Thoumine O
eLife (2020) 9: . 129 013 WB, IP; tested species: mouse

TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering.
Moretto E, Longatti A, Murru L, Chamma I, Sessa A, Zapata J, Hosy E, Sainlos M, Saint-Pol J, Rubinstein E, Choquet D, et al.
Cell reports (2019) 295: 1130-1146.e8. 129 013 WB; tested species: mouse

Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization.
Liu A, Zhou Z, Dang R, Zhu Y, Qi J, He G, Leung C, Pak D, Jia Z, Xie W
The Journal of cell biology (2016) 2124: 449-63. 129 013 WB; tested species: mouse

Synaptic Contacts Enhance Cell-to-Cell Tau Pathology Propagation.
Calafate S, Buist A, Miskiewicz K, Vijayan V, Daneels G, de Strooper B, de Wit J, Verstreken P, Moechars D
Cell reports (2015) 118: 1176-83. 129 013 WB; KD verified; tested species: rat

Promoter-like sequences regulating transcriptional activity in neurexin and neuroligin genes.
Runkel F, Rohlmann A, Reissner C, Brand SM, Missler M
Journal of neurochemistry (2013) 1271: 36-47. 129 013 WB

Cat. No.: 129 013

Amount: 50 µg

Price: $375.00

Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.

1 ) Song JY et al., Proc. Natl. Acad. Sci. U.S.A. (1999)
2 ) Missler M et al., J. Neurochem. (1998)
3 ) Ichtchenko K et al., J. Biol. Chem. (1996)
4 ) Ichtchenko K et al., Cell (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) Chubykin AA et al., Neuron (2007)
7 ) Chubykin AA et al., J. Biol. Chem. (2005)
8 ) Varoqueaux F et al., Eur. J. Cell Biol. (2004)
9 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)

Protocols

Standard protocol

Other products for this target

Neuroligin1 antibody cytoplasmic domain - 129 013 K.O.

References for Neuroligin1 - 129 013