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APP - 127 003

Protein involved in Alzheimer's disease
Polyclonal rabbit purified antibody
Cat. No.: 127 003
Amount: 50 µg
Price: 450.00 €
Cat. No. 127 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000      gallery  
IHC-P/FFPE: 1 : 2000      gallery  
Immunogen Synthetic peptide corresponding to AA 756 to 770 from rat APP (UniProt Id: P08592)
Reactivity Reacts with: rat (P08592), mouse (P12023), chicken, frog, human (P05067).
Other species not tested yet.
Specificity Specific for APP.
Matching control protein/peptide 127-0P
Data sheet 127_003.pdf

References for APP - 127 003

Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Surface Trafficking of APP and BACE in Live Cells.
Bauereiss A, Welzel O, Jung J, Grosse-Holz S, Lelental N, Lewczuk P, Wenzel EM, Kornhuber J, Groemer TW
Traffic (Copenhagen, Denmark) (2015) 166: 655-75. 127 003 ICC
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Transformation of diffuse beta-amyloid precursor protein and beta-amyloid deposits to plaques in the thalamus after transient occlusion of the middle cerebral artery in rats.
van Groen T, Puurunen K, Mäki HM, Sivenius J, Jolkkonen J
Stroke (2005) 367: 1551-6. 127 003 IHC; tested species: rat
The metalloprotease ADAMTS4 generates N-truncated Aβ4-x species and marks oligodendrocytes as a source of amyloidogenic peptides in Alzheimer's disease.
Walter S, Jumpertz T, Hüttenrauch M, Ogorek I, Gerber H, Storck SE, Zampar S, Dimitrov M, Lehmann S, Lepka K, Berndt C, et al.
Acta neuropathologica (2018) : . 127 003 IHC-P; tested species: mouse
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Cat. No.: 127 003
Quantity: 50 µg
Price: 450.00 €
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Surface Trafficking of APP and BACE in Live Cells.
Bauereiss A, Welzel O, Jung J, Grosse-Holz S, Lelental N, Lewczuk P, Wenzel EM, Kornhuber J, Groemer TW
Traffic (Copenhagen, Denmark) (2015) 166: 655-75. 127 003 ICC
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Transformation of diffuse beta-amyloid precursor protein and beta-amyloid deposits to plaques in the thalamus after transient occlusion of the middle cerebral artery in rats.
van Groen T, Puurunen K, Mäki HM, Sivenius J, Jolkkonen J
Stroke (2005) 367: 1551-6. 127 003 IHC; tested species: rat
The metalloprotease ADAMTS4 generates N-truncated Aβ4-x species and marks oligodendrocytes as a source of amyloidogenic peptides in Alzheimer's disease.
Walter S, Jumpertz T, Hüttenrauch M, Ogorek I, Gerber H, Storck SE, Zampar S, Dimitrov M, Lehmann S, Lepka K, Berndt C, et al.
Acta neuropathologica (2018) : . 127 003 IHC-P; tested species: mouse
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Background

Alzheimer's disease is characterized by the accumulation of β-amyloid peptides in plaques and vessel walls and by the intraneuronal accumulation of paired helical filaments composed of hyperphosphorylated tau.
Amyloid precursor protein APP is part of a super-family of transmembrane and secreted proteins. It appears to have a number of roles, including regulation of haemostasis and mediation of neuroprotection. APP also has metal and heparin-binding properties. Cleavage of amyloid precursor protein by β- and γ-secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents formation from APP.
Recent findings indicate that the site of γ-secretase cleavage is critical to the development of amyloid deposits. Aβ1-42 is much more amyloidogenic than Aβ1-40. Aβ1-42 formation is favoured by mutations in the two presenilin genes (PS1 and PS2), and by the commonest amyloid precursor protein mutations.