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Home > Products > 127 003

APP antibody - 127 003 K.O.

APP is a protein involved in Alzheimer's disease
Rabbit polyclonal purified antibody
Cat. No.: 127 003
Amount: 50 µg
Price: $455.00
Cat. No. 127 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000      gallery  
IHC-P: 1 : 2000      gallery  
DNA-PAINT: yes (see remarks)

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.
Immunogen Synthetic peptide corresponding to AA 756 to 770 from rat APP (UniProt Id: P08592)
Reactivity Reacts with: rat (P08592), mouse (P12023), chicken, frog, human (P05067).
Other species not tested yet.
Specificity Specific for APP K.O. validated
Matching control protein/peptide 127-0P
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).
Data sheet 127_003.pdf

References for APP - 127 003

Oligodendrocytes produce amyloid-β and contribute to plaque formation alongside neurons in Alzheimer's disease model mice.
Sasmita AO, Depp C, Nazarenko T, Sun T, Siems SB, Ong EC, Nkeh YB, Böhler C, Yu X, Bues B, Evangelista L, et al.
Nature neuroscience (2024) : . 127 003 WB; tested species: mouse
The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 127 003 WB, ICC; tested species: mouse
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 127 003 WB, ICC; tested species: mouse
Surface Trafficking of APP and BACE in Live Cells.
Bauereiss A, Welzel O, Jung J, Grosse-Holz S, Lelental N, Lewczuk P, Wenzel EM, Kornhuber J, Groemer TW
Traffic (Copenhagen, Denmark) (2015) 166: 655-75. 127 003 ICC
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Transformation of diffuse beta-amyloid precursor protein and beta-amyloid deposits to plaques in the thalamus after transient occlusion of the middle cerebral artery in rats.
van Groen T, Puurunen K, Mäki HM, Sivenius J, Jolkkonen J
Stroke (2005) 367: 1551-6. 127 003 IHC; tested species: rat
The metalloprotease ADAMTS4 generates N-truncated Aβ4-x species and marks oligodendrocytes as a source of amyloidogenic peptides in Alzheimer's disease.
Walter S, Jumpertz T, Hüttenrauch M, Ogorek I, Gerber H, Storck SE, Zampar S, Dimitrov M, Lehmann S, Lepka K, Berndt C, et al.
Acta neuropathologica (2018) : . 127 003 IHC-P; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 127 003 DNA-PAINT; tested species: rat
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Cat. No.: 127 003
Amount: 50 µg
Price: $455.00
Oligodendrocytes produce amyloid-β and contribute to plaque formation alongside neurons in Alzheimer's disease model mice.
Sasmita AO, Depp C, Nazarenko T, Sun T, Siems SB, Ong EC, Nkeh YB, Böhler C, Yu X, Bues B, Evangelista L, et al.
Nature neuroscience (2024) : . 127 003 WB; tested species: mouse
The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 127 003 WB, ICC; tested species: mouse
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
The amyloid precursor protein is a conserved Wnt receptor.
Liu T, Zhang T, Nicolas M, Boussicault L, Rice H, Soldano A, Claeys A, Petrova I, Fradkin L, De Strooper B, Potier MC, et al.
eLife (2021) 10: . 127 003 WB, ICC; tested species: mouse
Surface Trafficking of APP and BACE in Live Cells.
Bauereiss A, Welzel O, Jung J, Grosse-Holz S, Lelental N, Lewczuk P, Wenzel EM, Kornhuber J, Groemer TW
Traffic (Copenhagen, Denmark) (2015) 166: 655-75. 127 003 ICC
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
Transformation of diffuse beta-amyloid precursor protein and beta-amyloid deposits to plaques in the thalamus after transient occlusion of the middle cerebral artery in rats.
van Groen T, Puurunen K, Mäki HM, Sivenius J, Jolkkonen J
Stroke (2005) 367: 1551-6. 127 003 IHC; tested species: rat
The metalloprotease ADAMTS4 generates N-truncated Aβ4-x species and marks oligodendrocytes as a source of amyloidogenic peptides in Alzheimer's disease.
Walter S, Jumpertz T, Hüttenrauch M, Ogorek I, Gerber H, Storck SE, Zampar S, Dimitrov M, Lehmann S, Lepka K, Berndt C, et al.
Acta neuropathologica (2018) : . 127 003 IHC-P; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 127 003 DNA-PAINT; tested species: rat
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 127 003 WB, ICC, EM
WB
WB
ICC
IHC
IHC-P
Background

Alzheimer's disease is characterized by the accumulation of β-amyloid peptides in plaques and vessel walls and by the intraneuronal accumulation of paired helical filaments composed of hyperphosphorylated tau.
Amyloid precursor protein APP is part of a super-family of transmembrane and secreted proteins. It appears to have a number of roles, including regulation of haemostasis and mediation of neuroprotection. APP also has metal and heparin-binding properties. Cleavage of amyloid precursor protein by β- and γ-secretases results in the generation of the Aβ (βA4)peptide, whereas α-secretase cleaves within the Aβ sequence and prevents formation from APP.
Recent findings indicate that the site of γ-secretase cleavage is critical to the development of amyloid deposits. Aβ1-42 is much more amyloidogenic than Aβ1-40. Aβ1-42 formation is favoured by mutations in the two presenilin genes (PS1 and PS2), and by the commonest amyloid precursor protein mutations.
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