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Munc 13-1 - 126 113

Mammalian homologues of the C. elegans unc-13 gene essential for exocytosis
Polyclonal rabbit purified antibody
Cat. No.: 126 113
Amount: 50 µg
Price: 370.00 €
Cat. No. 126 113 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 364 to 469 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity Reacts with: mouse (Q4KUS2), rat (Q62768).
Other species not tested yet.
Specificity Specific for Munc13-1 K.O.
Data sheet 126_113.pdf
Cat. No.: 126 113
Quantity: 50 µg
Price: 370.00 €
Background
Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.