Synaptic Systems - Munc13-1

Cat. No.: 126 008

Amount: 50 µg

Price: $420.00

Munc13-1 (red) and PCP4 (green) staining in a mouse cerebellum section

WB

ICC

IHC

Background

Munc13s are homologues of the C. elegans unc-13 gene product. Three brain-specific isoforms, Munc 13-1, -13-2, and -13-3, are expressed in mammals, where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb, and hippocampus. Munc13-2 is mainly expressed in cortex and hippocampus, whereas Munc13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled-coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc13-2) has been described.
Munc13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Sheu L et al., J. Biol. Chem. (2003)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Ohtsuka T et al., J. Cell Biol. (2002)
4 ) Ashery U et al., EMBO J. (2000)
5 ) Brose N et al., Curr. Opin. Neurobiol. (2000)
6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

WB

ICC

Fixed Cells

IHC

Other products for this target

Cat. No. 126 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb147D6
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the N-terminus of rat Munc13-1 (UniProt Id: Q62768)
Reactivity	Reacts with: mouse (Q4KUS2), rat (Q62768). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-147D6. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	Datasheet 126_008

Munc13-1 antibody - 126 008