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Complexin4 antibody - 122 411 K.O.

Complexin4 is a nerve-terminal syntaxin binding protein in retinal ribbon synapses
Mouse monoclonal purified IgG
Cat. No.: 122 411
Amount: 100 µg
Price: $415.00
Cat. No. 122 411 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (ECL detection) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 2000 (see remarks) gallery  
IHC-P: 1 : 200 gallery  
Clone 171H8
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 160 from mouse Complexin4 (UniProt Id: Q80WM3)
Reactivity Reacts with: rat (D3ZM85), mouse (Q80WM3).
Other species not tested yet.
Specificity Specific for complexin 4, no cross reaction to other complexins. K.O. validated PubMed: 18505837
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.

Data sheet 122_411.pdf

References for Complexin4 - 122 411

Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 122 411 WB; KO verified; tested species: mouse
Functional roles of complexin in neurotransmitter release at ribbon synapses of mouse retinal bipolar neurons.
Vaithianathan T, Henry D, Akmentin W, Matthews G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 359: 4065-70. 122 411 ICC
Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex.
Lux UT, Meyer J, Jahn O, Davison A, Babai N, Gießl A, Wartenberg A, Sticht H, Brose N, Reim K, Brandstätter JH, et al.
Frontiers in molecular neuroscience (2024) 17: 1308466. 122 411 IHC; tested species: mouse
The absence of Complexin 3 and Complexin 4 differentially impacts the ON and OFF pathways in mouse retina.
Landgraf I, Mühlhans J, Dedek K, Reim K, Brandstätter JH, Ammermüller J
The European journal of neuroscience (2012) 364: 2470-81. 122 411 IHC
Cat. No.: 122 411
Amount: 100 µg
Price: $415.00
Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 122 411 WB; KO verified; tested species: mouse
Functional roles of complexin in neurotransmitter release at ribbon synapses of mouse retinal bipolar neurons.
Vaithianathan T, Henry D, Akmentin W, Matthews G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 359: 4065-70. 122 411 ICC
Light-dependent regulation of neurotransmitter release from rod photoreceptor ribbon synapses involves an interplay of Complexin 4 and Transducin with the SNARE complex.
Lux UT, Meyer J, Jahn O, Davison A, Babai N, Gießl A, Wartenberg A, Sticht H, Brose N, Reim K, Brandstätter JH, et al.
Frontiers in molecular neuroscience (2024) 17: 1308466. 122 411 IHC; tested species: mouse
The absence of Complexin 3 and Complexin 4 differentially impacts the ON and OFF pathways in mouse retina.
Landgraf I, Mühlhans J, Dedek K, Reim K, Brandstätter JH, Ammermüller J
The European journal of neuroscience (2012) 364: 2470-81. 122 411 IHC
Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2 is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.