|Cat. No. 122 402||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: not tested yet
ICC: not tested yet
IHC: 1 : 4000 up to 1 : 40000 gallery
IHC-P/FFPE: 1 : 200 gallery
|Immunogen||Recombinant protein corresponding to AA 1 to 160 from mouse Complexin4 (UniProt Id: Q80WM3)|
Reacts with: rat (D3ZM85), mouse (Q80WM3).
Other species not tested yet.
|Specificity||Specific for complexin 4, no cross reaction to other complexins. K.O. PubMed: 19386896|
Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2 is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.