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Complexin 4 - 122 402

A nerve-terminal syntaxin binding protein in retinal ribbon synapses
Polyclonal rabbit antiserum
Cat. No.: 122 402
Amount: 200 µl
Price: $350.00
Cat. No. 122 402 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 4000 up to 1 : 40000 gallery  
IHC-P/FFPE: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 1 to 160 from mouse Complexin4 (UniProt Id: Q80WM3)
Reactivity Reacts with: rat (D3ZM85), mouse (Q80WM3).
Other species not tested yet.
Specificity Specific for complexin 4, no cross reaction to other complexins. K.O. PubMed: 19386896
Data sheet 122_402.pdf

References for Complexin 4 - 122 402

Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 122 402 WB
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 402 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 402 WB, IHC; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 402 WB, IHC
Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell.
Bajohrs M, Darios F, Peak-Chew SY, Davletov B
The Biochemical journal (2005) 392Pt 2: 283-9. 122 402 WB
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 402 WB, ICC, IHC; tested species: zebrafish
Calcium channel-dependent molecular maturation of photoreceptor synapses.
Zabouri N, Haverkamp S
PloS one (2013) 85: e63853. 122 402 IHC
The absence of Complexin 3 and Complexin 4 differentially impacts the ON and OFF pathways in mouse retina.
Landgraf I, Mühlhans J, Dedek K, Reim K, Brandstätter JH, Ammermüller J
The European journal of neuroscience (2012) 364: 2470-81. 122 402 IHC
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 402 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 402 WB, IHC; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 402 WB, IHC
Cat. No.: 122 402
Quantity: 200 µl
Price: $350.00
Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 122 402 WB
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 402 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 402 WB, IHC; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 402 WB, IHC
Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell.
Bajohrs M, Darios F, Peak-Chew SY, Davletov B
The Biochemical journal (2005) 392Pt 2: 283-9. 122 402 WB
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 402 WB, ICC, IHC; tested species: zebrafish
Calcium channel-dependent molecular maturation of photoreceptor synapses.
Zabouri N, Haverkamp S
PloS one (2013) 85: e63853. 122 402 IHC
The absence of Complexin 3 and Complexin 4 differentially impacts the ON and OFF pathways in mouse retina.
Landgraf I, Mühlhans J, Dedek K, Reim K, Brandstätter JH, Ammermüller J
The European journal of neuroscience (2012) 364: 2470-81. 122 402 IHC
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 402 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 402 WB, IHC; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 402 WB, IHC
Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2 is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.