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Complexin 3 - 122 302

A nerve-terminal syntaxin binding protein in retinal ribbon synapses
Polyclonal rabbit antiserum
Cat. No.: 122 302
Amount: 200 µl
Price: $350.00
Cat. No. 122 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 10000 gallery  
IHC-P/FFPE: 1 : 200 gallery  
Immunogen Recombinant protein corresponding to AA 1 to 158 from mouse Complexin3 (UniProt Id: Q8R1B5)
Reactivity Reacts with: rat (D4ABY0), mouse (Q8R1B5).
Other species not tested yet.
Specificity Specific for complexin 3, no cross reaction to other complexins. K.O. PubMed: 18505837
Data sheet 122_302.pdf

References for Complexin 3 - 122 302

Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 122 302 WB
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 122 302 WB, ICC; tested species: rat
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 302 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 302 WB, IHC; KO verified; tested species: mouse
Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 122 302 WB; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 302 WB, IHC
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 122 302 WB, ICC; tested species: rat
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 302 WB, ICC, IHC; tested species: zebrafish
Altered White Matter and Layer VIb Neurons in Heterozygous Disc1 Mutant, a Mouse Model of Schizophrenia.
Tsai SH, Tsao CY, Lee LJ
Frontiers in neuroanatomy (2020) 14: 605029. 122 302 IHC; tested species: mouse
Kcnab1 Is Expressed in Subplate Neurons With Unilateral Long-Range Inter-Areal Projections.
Tiong SYX, Oka Y, Sasaki T, Taniguchi M, Doi M, Akiyama H, Sato M
Frontiers in neuroanatomy (2019) 13: 39. 122 302 IHC; tested species: mouse
Neonatal Hypoxia-Ischemia Causes Functional Circuit Changes in Subplate Neurons.
Sheikh A, Meng X, Liu J, Mikhailova A, Kao JPY, McQuillen PS, Kanold PO
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 122 302 IHC; tested species: mouse
Unbiased Quantification of Subplate Neuron Loss following Neonatal Hypoxia-Ischemia in a Rat Model.
Mikhailova A, Sunkara N, McQuillen PS
Developmental neuroscience (2017) 391-4: 171-181. 122 302 IHC; tested species: rat
Transient Hypoxemia Chronically Disrupts Maturation of Preterm Fetal Ovine Subplate Neuron Arborization and Activity.
McClendon E, Shaver DC, Degener-O'Brien K, Gong X, Nguyen T, Hoerder-Suabedissen A, Molnár Z, Mohr C, Richardson BD, Rossi DJ, Back SA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3749: 11912-11929. 122 302 IHC; tested species: mouse
Allelic specificity of Ube3a expression in the mouse brain during postnatal development.
Judson MC, Sosa-Pagan JO, Del Cid WA, Han JE, Philpot BD
The Journal of comparative neurology (2014) 5228: 1874-96. 122 302 IHC; tested species: mouse
Extracortical origin of some murine subplate cell populations.
Pedraza M, Hoerder-Suabedissen A, Albert-Maestro MA, Molnár Z, De Carlos JA
Proceedings of the National Academy of Sciences of the United States of America (2014) 11123: 8613-8. 122 302 IHC; tested species: mouse
Calcium channel-dependent molecular maturation of photoreceptor synapses.
Zabouri N, Haverkamp S
PloS one (2013) 85: e63853. 122 302 IHC
Changing microcircuits in the subplate of the developing cortex.
Viswanathan S, Bandyopadhyay S, Kao JP, Kanold PO
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 325: 1589-601. 122 302 IHC; tested species: mouse
Early-onset, slow progression of cone photoreceptor dysfunction and degeneration in CNG channel subunit CNGB3 deficiency.
Xu J, Morris L, Fliesler SJ, Sherry DM, Ding XQ
Investigative ophthalmology & visual science (2011) 526: 3557-66. 122 302 IHC; tested species: mouse
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 302 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 302 WB, IHC; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 302 WB, IHC
Cat. No.: 122 302
Quantity: 200 µl
Price: $350.00
Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 122 302 WB
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 122 302 WB, ICC; tested species: rat
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 302 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 302 WB, IHC; KO verified; tested species: mouse
Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
Xue M, Stradomska A, Chen H, Brose N, Zhang W, Rosenmund C, Reim K
Proceedings of the National Academy of Sciences of the United States of America (2008) 10522: 7875-80. 122 302 WB; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 302 WB, IHC
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 122 302 WB, ICC; tested species: rat
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 302 WB, ICC, IHC; tested species: zebrafish
Altered White Matter and Layer VIb Neurons in Heterozygous Disc1 Mutant, a Mouse Model of Schizophrenia.
Tsai SH, Tsao CY, Lee LJ
Frontiers in neuroanatomy (2020) 14: 605029. 122 302 IHC; tested species: mouse
Kcnab1 Is Expressed in Subplate Neurons With Unilateral Long-Range Inter-Areal Projections.
Tiong SYX, Oka Y, Sasaki T, Taniguchi M, Doi M, Akiyama H, Sato M
Frontiers in neuroanatomy (2019) 13: 39. 122 302 IHC; tested species: mouse
Neonatal Hypoxia-Ischemia Causes Functional Circuit Changes in Subplate Neurons.
Sheikh A, Meng X, Liu J, Mikhailova A, Kao JPY, McQuillen PS, Kanold PO
Cerebral cortex (New York, N.Y. : 1991) (2018) : . 122 302 IHC; tested species: mouse
Unbiased Quantification of Subplate Neuron Loss following Neonatal Hypoxia-Ischemia in a Rat Model.
Mikhailova A, Sunkara N, McQuillen PS
Developmental neuroscience (2017) 391-4: 171-181. 122 302 IHC; tested species: rat
Transient Hypoxemia Chronically Disrupts Maturation of Preterm Fetal Ovine Subplate Neuron Arborization and Activity.
McClendon E, Shaver DC, Degener-O'Brien K, Gong X, Nguyen T, Hoerder-Suabedissen A, Molnár Z, Mohr C, Richardson BD, Rossi DJ, Back SA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3749: 11912-11929. 122 302 IHC; tested species: mouse
Allelic specificity of Ube3a expression in the mouse brain during postnatal development.
Judson MC, Sosa-Pagan JO, Del Cid WA, Han JE, Philpot BD
The Journal of comparative neurology (2014) 5228: 1874-96. 122 302 IHC; tested species: mouse
Extracortical origin of some murine subplate cell populations.
Pedraza M, Hoerder-Suabedissen A, Albert-Maestro MA, Molnár Z, De Carlos JA
Proceedings of the National Academy of Sciences of the United States of America (2014) 11123: 8613-8. 122 302 IHC; tested species: mouse
Calcium channel-dependent molecular maturation of photoreceptor synapses.
Zabouri N, Haverkamp S
PloS one (2013) 85: e63853. 122 302 IHC
Changing microcircuits in the subplate of the developing cortex.
Viswanathan S, Bandyopadhyay S, Kao JP, Kanold PO
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 325: 1589-601. 122 302 IHC; tested species: mouse
Early-onset, slow progression of cone photoreceptor dysfunction and degeneration in CNG channel subunit CNGB3 deficiency.
Xu J, Morris L, Fliesler SJ, Sherry DM, Ding XQ
Investigative ophthalmology & visual science (2011) 526: 3557-66. 122 302 IHC; tested species: mouse
Enrichment and differential targeting of complexins 3 and 4 in ribbon-containing sensory neurons during zebrafish development.
Zanazzi G, Matthews G
Neural development (2010) 5: 24. 122 302 WB, ICC, IHC; tested species: zebrafish
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 122 302 WB, IHC; KO verified; tested species: mouse
Structurally and functionally unique complexins at retinal ribbon synapses.
Reim K, Wegmeyer H, Brandstätter JH, Xue M, Rosenmund C, Dresbach T, Hofmann K, Brose N
The Journal of cell biology (2005) 1694: 669-80. 122 302 WB, IHC
Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2 is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.